ibidi Solutions for Virology Research

ibidi offers several specialized products that are ideal for analyzing the impact of viruses on cells. In addition, as a consequence of the current COVID-19 pandemic, the establishment of physiologic lung models are of critical importance. Since March 2020, more than 20 papers using ibidi products for COVID-19 research have been published. Below, you will find relevant ibidi solutions and related publications in this line of research.

We are looking for collaborators: please contact us at [email protected],
if you would like to cooperate with ibidi on a specific application.

ibidi Products for Virology Research:

µ-Slide Membrane ibiPore Flow

  • A µ-Slide with a porous glass membrane, suitable for the establishment of lung models with air-liquid interface (ALI)

Find more details and ordering information here.


The μ-Slide Membrane ibiPore Flow consists of a horizontal porous glass membrane that is inserted between two channels. The upper channel is a static reservoir above the membrane. The lower channel is a perfusion channel for applying defined shear stress on cells, which are attached to the membrane. The upper and the lower channel communicate with each other only across the membrane.

S_850XX_ibiPore_Flow_grafik_01.jpg

ibiPore_01.jpg

Lung Models with Air-Liquid Interface (ALI)

To create a lung model with an air-liquid interface (ALI), a polarized cell monolayer (e.g., lung epithelial cells) is cultured on one side of the porous membrane that is exposed to air. From the lower channel, the cells are supplied by culture medium under defined shear stress.

S_850XX_ibiPore_Flow_appl_ex6.jpg



Phase contrast microscopy of A549 lung cells cultured on the µ-Slide Membrane ibiPore Flow on an ALI. A549 lung cells (adenocarcinomic human alveolar basal epithelial cells) were seeded on the upper side of the membrane and the lower channel was filled with culture medium. After cell adherence, the culture medium in the upper channel was replaced with air to establish an air-liquid interface. For imaging, the air was replaced with cell culture medium. Pore size 8 µm, 5% porosity. Data by Dr. Anita Reiser, Chair of Prof. Rädler, Ludwig-Maximilians-University Munich.


Cells on upper side of the membrane


Cells on lower side of the membrane

Phase contrast microscopy of A549 lung cells (adenocarcinomic human alveolar basal epithelial cells) cultured on the µ-Slide Membrane ibiPore Flow, pore size 3 µm, 5% porosity. Left: cells were seeded on the upper side of the membrane. Right: cells were seeded on the lower side of the membrane. Data by Dr. Anita Reiser, Chair of Prof. Rädler, Ludwig-Maximilians-University Munich.

µ-Slide III 3D Perfusion

  • A µ-Slide with three channels, ideal for the long-term cultivation of cells and tissues (e.g., lung tissue from biopsies) in 3D matrices with an optimal nutrient supply

Find more details and ordering information here.

S_8037X_III_3D_Perfusion_RGB_Icon_263.jp

µ-Slide 8 Well

  • A chambered coverslip with 8 wells for everyday cell culture, immunofluorescence, and high-end microscopy; suitable for a variety of virology-related assays

Find more details and ordering information here.

S_8082X_Slide_8well_RGB_2Icons.jpg

Selected References

S. Bauernfried, M.J. Scherr, A. Pichlmair, K.E. Duderstadt, V. Hornung. Human NLRP1 is a sensor for double-stranded RNA. Science, 2020, 10.1126/science.abd0811.
Read article

O. O. Onabajo, A. R. Banday, M. L. Stanifer, W. Yan, A. Obajemu, D. M. Santer, O. Florez-Vargas, H. Piontkivska, J. M. Vargas, T. J. Ring, C. Kee, P. Doldan, D. L. Tyrrell, J. L. Mendoza, S. Boulant and L. Prokunina-Olsson. Interferons and viruses induce a novel truncated ACE2 isoform and not the full-length SARS-CoV-2 receptor. Nature Genetics, 2020, 10.1038/s41588-020-00731-9.
Read article

M. L. Stanifer, C. Kee, M. Cortese, C. Zumaran, S. Triana, M. Mukenhirn, H.-G. Kraeusslich, T. Alexandrov, R. Bartenschlager and S. Boulant. Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells. Cell Reports, 2020, 10.1016/j.celrep.2020.107863.
Read article

C. Conzelmann, R. Groß, M. Zou, F. Krüger, A. Görgens, M. O. Gustafsson, S. El Andaloussi, J. Münch and J. A. Müller. Salivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection. Journal of Extracellular Vesicles, 2020, 10.1080/20013078.2020.1808281
Read article

Bohr, N. Tsapis, C. Foged, I. Andreana, M. Yang and E. Fattal. Treatment of acute lung inflammation by pulmonary delivery of anti-TNF-α siRNA with PAMAM dendrimers in a murine model. European Journal of Pharmaceutics and Biopharmaceutics, 2020, 10.1016/j.ejpb.2020.08.009.
Read article

L. Wendt, J. Brandt, B. Bodmer, S. Reiche, M.L. Schmidt, S. Traeger and T. Hoenen. The Ebola Virus Nucleoprotein Recruits the Nuclear RNA Export Factor NXF1 into Inclusion Bodies to Facilitate Viral Protein Expression. Cells, 2020, 10.3390/cells9010187.
Read article

J.K. Millet, F. Kien, C.Y. Cheung, Y.L. Siu, W.L. Chan, H. Li, H.L. Leung, M. Jaume, R. Bruzzone, J.S. Peiris, R.M. Altmeyer and B. Nal. Ezrin interacts with the SARS coronavirus Spike protein and restrains infection at the entry stage. PLoS One, 2012, 10.1371/journal.pone.0049566.
Read article

S. Pfefferle, V. Krähling, V. Ditt, K. Grywna, E. Mühlberger and C. Drosten . Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo. Virology Journal, 2009, 10.1186/1743-422X-6-131.
Read article

Glass Bottom Dish 35 mm

  • A 35 mm cell imaging dish with a glass bottom for everyday cell culture and fluorescence microscopy

Find more details and ordering information here.

Selected Reference

W.B. Klimstra, N.L. Tilston-Lunel, S. Nambulli, J.Boslett, C.M. McMillen, T. Gilliland, M.D. Dunn, C. Sun, S. E. Wheeler, A. Wells, A.L. Hartman, A.K. McElroy, D.S. Reed, L.J. Rennick and W. P. Duprex. SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected hospitalized COVID-19 patients. Journal of General Virology, 2020, 10.1099/jgv.0.001481
Read article

D_81218_GB_Dish_35mm_RGB_Icon_263.jpg

µ-Dish 35 mm Quad

  • A 35 mm imaging dish with four compartments and the ibidi Polymer Coverslip Bottom—for simultaneous cellular assays and high end microscopy

Find more details and ordering information here.

Selected Reference

J. Buchrieser, J. Dufloo, M. Hubert, B. Monel, D. Planas, M. M. Rajah, C. Planchais, F. Porrot, F. Guivel-Benhassine, S. Van der Werf, N. Casartelli, H. Mouquet, T. Bruel and O. Schwartz. Syncytia formation by SARS-CoV-2-infected cells. EMBO J., 2020, 10.15252/embj.2020106267.
Read article

D_8041X_35mm_Quad_RGB_Icon_263.jpg

12 Well Chamber, removable

  • Chamber slide with a removable 12 well silicone chamber for cell culture and immunofluorescence staining

Find more details and ordering information here.

Selected Reference

T. Thi Nhu Thao, F. Labroussaa, N. Ebert, P. V’kovski, H. Stalder, J. Portmann, J. Kelly, S. Steiner, M. Holwerda, A. Kratzel, M. Gultom, K. Schmied, L. Laloli, L. Hüsser, M. Wider, S. Pfaender, D. Hirt, V. Cippà, S. Crespo-Pomar, S. Schröder, D. Muth, D. Niemeyer, V. M. Corman, M. A. Müller, C. Drosten, R. Dijkman, J. Jores and V. Thiel. Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform. Nature, 2020, 10.1038/s41586-020-2294-9
Read article

S_81201_12Well_Removable_RGB_Icon_263.jpg

µ-Slide VI 0.1

  • A six channel µ-Slide for parallel flow assays and immunofluorescence that uses a minimal amount of reagents and medium

Find more details and ordering information here.

Selected Reference

Robb and colleagues have recently published a novel single-particle tracking assay for a variety of enveloped viruses using the ibidi µ-Slide VI 0.1. This assay helps to detect and count viruses via fluorescence microscopy after just adding a mixture of DNA, CaCl2, and fluorophores to the virus. The procedure even works at low titers, which are present in clinical samples.
N.C. Robb, J.M. Taylor, A. Kent, O.J. Pambos, B. Gilboa and A.N. Kapanidis. Rapid functionalisation and detection of viruses via a novel Ca2+-mediated virus-DNA interaction. Scientific Reports, 2019, 10.1101/629303.
Read article

S_8066X_Slide_VI01_RGB_Icon_263.jpg

µ-Slide VI 0.4

  • A six channel µ-Slide suitable for flow experiments and for immunofluorescence assays

Find more details and ordering information here.

Selected Reference

L. Nicolai, A. Leunig, S. Brambs, R. Kaiser, T. Weinberger, M. Weigand, M. Muenchhoff, J.C. Hellmuth, S. Ledderose, H. Schulz, C. Scherer, M. Rudelius, M. Zoller, D. Höchter, O. Keppler, D. Teupser, B. Zwißler, M. von Bergwelt-Baildon, S. Kääb, S. Massberg, K. Pekayvaz and K. Stark. Immunothrombotic dysregulation in COVID-19 pneumonia is associated with respiratory failure and coagulopathy. Circulation, 2020, 10.1161/CIRCULATIONAHA.120.048488.
Read article

S_8060X_Slide_VI04_RGB_Icon_263.jpg

µ-Plate 96 Well Black

  • A black 96 well plate with square and flat and clear bottom for high throughput applications in cell-based assays

Find more details and ordering information here.

µPlate 96 Well Black

Selected Reference

S. Bauernfried, M.J. Scherr, A. Pichlmair, K.E. Duderstadt, V. Hornung. Human NLRP1 is a sensor for double-stranded RNA. Science, 2020, 10.1126/science.abd0811.
Read article