ibidi Solutions for Virology Research
ibidi offers several specialized products that are ideal for analyzing the impact of viruses on cells. In addition, as a consequence of the current COVID-19 pandemic, the establishment of physiologic lung models are of critical importance. Since March 2020, hundreds of papers using ibidi products for COVID-19 research have been published. Below, you will find relevant ibidi solutions and related publications in this line of research.
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if you would like to cooperate with ibidi on a specific application.
ibidi Products for Virology Research:
- A µ-Slide with three channels, ideal for the long-term cultivation of cells and tissues (e.g., lung tissue from biopsies) in 3D matrices with an optimal nutrient supply
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- A chambered coverslip with 8 wells for everyday cell culture, immunofluorescence, and high-end microscopy; suitable for a variety of virology-related assays
Biopsychronology of a COVID-19 patient blood sample in the ibidi μ-Slide 8 Well after induction of coagulation. The right side shows the area of the blood clot with the fibrin network (blue) and the erythrocytes (red). The abundant granulocytes that recognize and bind the invading viruses are visualized in green. Image acquisition was performed on a spinning disc confocal microscope.
Courtesy M. Bachler, M. Hermann and D. Fries, Department of Anesthesiology and Critical Care Medicine, Medical University Innsbruck, Austria.
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Selected References
S. Bauernfried, M.J. Scherr, A. Pichlmair, K.E. Duderstadt, V. Hornung. Human NLRP1 is a sensor for double-stranded RNA. Science, 2020, 10.1126/science.abd0811.
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O. O. Onabajo, A. R. Banday, M. L. Stanifer, W. Yan, A. Obajemu, D. M. Santer, O. Florez-Vargas, H. Piontkivska, J. M. Vargas, T. J. Ring, C. Kee, P. Doldan, D. L. Tyrrell, J. L. Mendoza, S. Boulant and L. Prokunina-Olsson. Interferons and viruses induce a novel truncated ACE2 isoform and not the full-length SARS-CoV-2 receptor. Nature Genetics, 2020, 10.1038/s41588-020-00731-9.
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M. L. Stanifer, C. Kee, M. Cortese, C. Zumaran, S. Triana, M. Mukenhirn, H.-G. Kraeusslich, T. Alexandrov, R. Bartenschlager and S. Boulant. Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells. Cell Reports, 2020, 10.1016/j.celrep.2020.107863.
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C. Conzelmann, R. Groß, M. Zou, F. Krüger, A. Görgens, M. O. Gustafsson, S. El Andaloussi, J. Münch and J. A. Müller. Salivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection. Journal of Extracellular Vesicles, 2020, 10.1080/20013078.2020.1808281
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Bohr, N. Tsapis, C. Foged, I. Andreana, M. Yang and E. Fattal. Treatment of acute lung inflammation by pulmonary delivery of anti-TNF-α siRNA with PAMAM dendrimers in a murine model. European Journal of Pharmaceutics and Biopharmaceutics, 2020, 10.1016/j.ejpb.2020.08.009.
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L. Wendt, J. Brandt, B. Bodmer, S. Reiche, M.L. Schmidt, S. Traeger and T. Hoenen. The Ebola Virus Nucleoprotein Recruits the Nuclear RNA Export Factor NXF1 into Inclusion Bodies to Facilitate Viral Protein Expression. Cells, 2020, 10.3390/cells9010187.
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J.K. Millet, F. Kien, C.Y. Cheung, Y.L. Siu, W.L. Chan, H. Li, H.L. Leung, M. Jaume, R. Bruzzone, J.S. Peiris, R.M. Altmeyer and B. Nal. Ezrin interacts with the SARS coronavirus Spike protein and restrains infection at the entry stage. PLoS One, 2012, 10.1371/journal.pone.0049566.
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S. Pfefferle, V. Krähling, V. Ditt, K. Grywna, E. Mühlberger and C. Drosten . Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo. Virology Journal, 2009, 10.1186/1743-422X-6-131.
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- A 35 mm cell imaging dish with a glass bottom for everyday cell culture and fluorescence microscopy
Selected Reference
W.B. Klimstra, N.L. Tilston-Lunel, S. Nambulli, J.Boslett, C.M. McMillen, T. Gilliland, M.D. Dunn, C. Sun, S. E. Wheeler, A. Wells, A.L. Hartman, A.K. McElroy, D.S. Reed, L.J. Rennick and W. P. Duprex. SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected hospitalized COVID-19 patients. Journal of General Virology, 2020, 10.1099/jgv.0.001481
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- A 35 mm imaging dish with four compartments and the ibidi Polymer Coverslip Bottom—for simultaneous cellular assays and high end microscopy
Selected Reference
J. Buchrieser, J. Dufloo, M. Hubert, B. Monel, D. Planas, M. M. Rajah, C. Planchais, F. Porrot, F. Guivel-Benhassine, S. Van der Werf, N. Casartelli, H. Mouquet, T. Bruel and O. Schwartz. Syncytia formation by SARS-CoV-2-infected cells. EMBO J., 2020, 10.15252/embj.2020106267.
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- Chamber slide with a removable 12 well silicone chamber for cell culture and immunofluorescence staining
Selected Reference
T. Thi Nhu Thao, F. Labroussaa, N. Ebert, P. V'kovski, H. Stalder, J. Portmann, J. Kelly, S. Steiner, M. Holwerda, A. Kratzel, M. Gultom, K. Schmied, L. Laloli, L. Hüsser, M. Wider, S. Pfaender, D. Hirt, V. Cippà, S. Crespo-Pomar, S. Schröder, D. Muth, D. Niemeyer, V. M. Corman, M. A. Müller, C. Drosten, R. Dijkman, J. Jores and V. Thiel. Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform. Nature, 2020, 10.1038/s41586-020-2294-9
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- A six channel µ-Slide for parallel flow assays and immunofluorescence that uses a minimal amount of reagents and medium
Selected Reference
Robb and colleagues have recently published a novel single-particle tracking assay for a variety of enveloped viruses using the ibidi µ-Slide VI 0.1. This assay helps to detect and count viruses via fluorescence microscopy after just adding a mixture of DNA, CaCl2, and fluorophores to the virus. The procedure even works at low titers, which are present in clinical samples.
N.C. Robb, J.M. Taylor, A. Kent, O.J. Pambos, B. Gilboa and A.N. Kapanidis. Rapid functionalisation and detection of viruses via a novel Ca2+-mediated virus-DNA interaction. Scientific Reports, 2019, 10.1101/629303.
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- A six channel µ-Slide suitable for flow experiments and for immunofluorescence assays
Selected Reference
L. Nicolai, A. Leunig, S. Brambs, R. Kaiser, T. Weinberger, M. Weigand, M. Muenchhoff, J.C. Hellmuth, S. Ledderose, H. Schulz, C. Scherer, M. Rudelius, M. Zoller, D. Höchter, O. Keppler, D. Teupser, B. Zwißler, M. von Bergwelt-Baildon, S. Kääb, S. Massberg, K. Pekayvaz and K. Stark. Immunothrombotic dysregulation in COVID-19 pneumonia is associated with respiratory failure and coagulopathy. Circulation, 2020, 10.1161/CIRCULATIONAHA.120.048488.
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- A bottomless slide with six channels and a self-adhesive underside that allows the mounting of a variety of bottom materials
Selected Reference
N. Furth , S. Shilo , N. Cohen , N. Erez, V. Fedyuk, A. M. Schrager, A. Weinberger, A. A. Dror, A. Zigron, M. Shehadeh, E. Sela, S. Srouji, S. Amit, I. Levy, E. Segal, R. Dahan, D. Jones, D. C. Douek, E. Shema. Unified platform for genetic and serological detection of COVID-19 with single-molecule technology. PLOS ONE, 2021, 10.1371/journal.pone.0255096.
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- A black 96 well plate with square and flat and clear bottom for high throughput applications in cell-based assays
Selected Reference
S. Bauernfried, M.J. Scherr, A. Pichlmair, K.E. Duderstadt, V. Hornung. Human NLRP1 is a sensor for double-stranded RNA. Science, 2020, 10.1126/science.abd0811.
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Find more details and ordering information here.
- A Stage Top Incubator for easy live cell imaging on every inverted microscope that has a multiwell plate holder or a K-frame
Selected Reference
E. Clough, K.T. Chean, J. Inigo, K.E. Tubbesing, D. Chandra, L. Chaves, J.L. Reynolds, R. Aalinkeel, S.A. Schwartz, A. Khmaladze, S.D. Mahajan. Mitochondrial Dynamics in SARS-COV2 Spike Protein Treated Human Microglia: Implications for Neuro-COVID. J Neuroimmune Pharmacol. 2021, 10.1007/s11481-021-10015-6. Epub ahead of print. PMID: 34599743; PMCID: PMC8487226.
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