Cell Culture Under Flow

Course Objectives

Level: Beginner

This course is based on the biological knowledge that several adherent cell types, such as endothelial cells in a blood vessel, are exposed to shear stress in vivo. Culturing cells in vitro under perfusion simulates this mechanical stimulus and induces a more physiological behavior.

This course will cover all aspects of setting up experiments with cells under flow. You will learn which critical factors need to be considered when conducting flow experiments. These include various physical parameters like shear rate, flow rate, viscosity, and shear stress calculations. We will also demonstrate helpful tips and tricks for the practical handling of ibidi Channel Slides.

After the course, you will be equipped with advanced knowledge and a better understanding of the necessary tools to perform your flow experiments like an expert.

No sterile working benches or cells are required to participate. Attend the course from the comfort of your office or home.

Target Group

This course is intended for scientists and technical associates who have experience with cell culture and sterile working techniques, and want to establish cell culture under flow experiments using ibidi Channel Slides.



New dates will be available soon. Please visit again later.


Day 1


  • Introduction to cell culture under flow
  • Physical background of liquid flow in channels
  • Tips and tricks for handling ibidi Channel Slides

Day 2


  • Tips and tricks for setting up a flow experiment in the lab
  • Assays for special flow applications
  • Discussion about your own experimental aims and setups

What Our Participants Say

"I participated in ibidi’s Cell Culture Under Flow online course and can confirm that it covered the most critical parameters for any researcher willing to start working on cell culture under flow experiments. The organizer’s communication skills, the quality of the slides, and the use of several interactive tools made the course very easy and enjoyable to follow. There was a great and very interactive atmosphere among the participants. For me, it was very important to gain an overview of the ibidi portfolio and learn how to make the best use of it with many good tips and tricks. I would recommend this course to all my colleagues."

Jorge Carvalho, Gulbenkian Science, Oeiras, Portugal

"Thank you for the online pump course. It was very interesting, even though we don't have the ibidi Pump System yet. I enjoyed it very much and, together with the introductory online course Cell Culture Under Flow, I got a very good overview of the application of shear stress on cells using the ibidi Pump System."

Paola Ciceri, University of Milan, Milan, Italy

"The ibidi Online Course Cell Culture Under Flow was very interesting, educational, and practical. You learn a lot and it was very enjoyable. I like the organization of the course and the content."

Laura Porres, University of Santiago de Compostela, Santiago de Compostela, Spain

"The ibidi Online Course Cell Culture Under Flow was really informative. It was packed with practical insights into using the slides and the Pump System, which I found incredibly valuable. The course also had a great interactive vibe. Questions were encouraged, and the discussions that followed were engaging. The course materials were well prepared and provided a solid reference for future use. The organizers put a lot of thought and effort into making sure we had everything we needed to continue learning after the course. All in all, I highly recommend this course if you're interested in cellular biology and flow systems."

Vinayak Sivaramakrishnan, Max Planck Institute for Molecular Biomedicine, Münster, Germany

"The Online Course Cell Culture Under Flow was super helpful as the concepts were made easy to understand even for researchers who are new to this field. The explanation for the physics of the system and the system itself was very detailed. Thank you for the course! I hope to attend some more informational courses in the future."

Renita Saldanha, Syracuse University, Syracuse, NY, USA