My collagen 3D gel looks very inhomogeneous. What is the reason for that?

Generally, an inhomogeneous appearance of the collagen gel is caused by starting polymerization too early. This can happen either due to insufficient cooling or incomplete mixing of the collagen solution. We recommend cooling the reagents you are using and keeping them on ice while preparing the 3D gel solution. We further recommend mixing the viscous collagen solution thoroughly before gel preparation.

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