Can water immersion objectives be used with the ibidi Mounting Medium (#50001)?
Ideally, objective lenses and mounting and immersion media should have similar refractive indices. The ibidi Mounting Medium has a refractive index of 1.515 and meets the standards for the highest optical performance with oil immersion lenses the best optical performance with oil immersion lenses. A mismatch of the objective lenses' refractive index and mounting medium can cause the loss of image resolution and signal intensity. Therefore, we recommend requesting a free sample of the ibidi Mounting Medium, then testing it with a water immersion objective to see if it achieves the required image quality.
Please note: the water immersion objectives are different from the water dipping lenses, which are used without a coverglass.
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