Parameters for Live Cell Imaging


The environmental temperature strongly influences the metabolism and the activity of cells. Temperature changes affect cell adhesion, protein expression, proliferation, and many more cellular parameters.

Generally, 37°C (which is our normal body temperature) is optimal for mammalian cell culture, including human, mouse, and rat cells. Cells from non-mammalian organisms, such as chick or yeast, require different temperatures.

In order to get robust and reliable results, and to maintain focus stability, the temperature of the cells and their direct environment must be kept as constant as possible during both short-term and long-term live cell imaging experiments.


Temperature development over time after switching on the ibidi Stage Top Incubator. The set temperature is reached quickly and remains stable over time.

ibidi Solution

The ibidi Stage Top Incubation Systems provide a stable and homogeneous temperature for live cell imaging experiments. The temperature is smoothly changeable and can easily be adapted to the requirements of the investigated cell type.



Stable and consistent temperature distribution in every well of multiwell plates in the ibidi Stage Top Incubation System. Images were acquired with a FLIR thermal camera.

Read on and learn more about the Carbon Dioxide (CO2) Levels in live cell imaging.