For pricing and online shopping, please log in.

Top sellers

µ-Slide 8 Well Glass Bottom

µ-Slide 8 Well Glass Bottom View larger

Open µ-Slide with 8 wells with a glass bottom, suitable for use in TIRF and single molecule applications

  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
  • Cost-effective experiments using small numbers of cells and low volumes of reagents

More details

#1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
15

Buy 4 - Get 1 Free

Quantity Discount
5 20%

Product Details

Applications:

  • Cultivation and microscopy of cell cultures
  • TIRF and single molecule applications

Technical Features

Specifications:

Number of wells 8
Dimensions of wells
(w x l x h) in mm
9.4 x 10.7 x 6.8
Volume per well 300 µl
Total height with lid 8 mm
Growth area per well 1.0 cm²
Coating area per well 2.20 cm²
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm



ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatings Compatible Compatible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Applications Fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately

Supporting Material

User Comments

Jessica Davis, University College Dublin, Ireland

"I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment — I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”

Jessica Davis
University College Dublin
Ireland

http://www.ucd.ie/

11 Related Products