µ-Slide 8 Well Glass Bottom

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µ-Slide 8 Well Glass Bottom

Open µ-Slide with 8 wells with a glass bottom, suitable for use in TIRF and single molecule applications

Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass

Cost-effective experiments using small numbers of cells and low volumes of reagents
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Open µ-Slide with 8 wells with a glass bottom, suitable for use in TIRF and single molecule applications

Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
Cost-effective experiments using small numbers of cells and low volumes of reagents

More details

Surface Modification

#1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized

Pcs./Box

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Quantity	Discount
5	20%

Product Details

Applications:

Cultivation and microscopy of cell cultures
TIRF and single molecule applications

Technical Features

Open µ-Slide with 8 independent wells
Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
May require coating to promote cell attachment
Also available as a µ-Slide 8 Well with an ibidi Polymer Coverslip Bottom for superior cell growth

Specifications:

Number of wells	8
Dimensions of wells (w x l x h) in mm	9.4 x 10.7 x 6.8
Volume per well	300 µl
Total height with lid	8 mm
Growth area per well	1.0 cm²
Coating area per well	2.20 cm²
Bottom:	Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

ibidi Polymer Coverslip vs. ibidi Glass Bottom

	ibidi Polymer Coverslip	ibidi Glass Bottom
Optical properties
Refractive index (n_D 589 nm)	1.52	1.52
Abbe number	56	55
Thickness	#1.5 (180 µm)	#1.5H (170 µm)
Material	Microscopy plastic	D 263M Schott borosilicate glass
Autofluorescence	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)
Birefringence (DIC)	Low (DIC compatible*)	Low (DIC compatible*)

Other aspects
Surface modifications	ibiTreat – tissue culture treated Uncoated – hydrophobic	Only glass
Protein coatings	Compatible	Compatible
Gas permeable	Yes	No
Material flexibility	High	Low
Breakable	No	Yes
Applications	Fluorescence microscopy	TIRF and single photon

* Special glass DIC Lids are available separately

Supporting Material

User Comments

Jessica Davis, University College Dublin, Ireland

"I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment — I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”

Jessica Davis
University College Dublin
Ireland

http://www.ucd.ie/