Single cells and spheroids do not attach to Bioinert. Does this mean that the spheroids can swim off the surface during imaging? If yes, how can this be avoided?

Even without cell attachment, the spheroids or clusters are positioned on the Bioinert surface in a stable way. When disturbing effects like strong convection, evaporation, or fast stage accelerations are not present, then there is no need to stabilize your cell samples on Bioinert. However, if a sample immobilization is necessary, we recommend increasing the viscosity of the culture medium (e.g., by using low melting agarose). We provide a detailed protocol in the Instructions (PDF).

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