How do you keep the cells in focus during a Matrigel™ assay?
Keeping the focus while taking images in time-lapse mode can be challenging. We have tested many methods, but have found that only autofocus helps.
3D Cell Culture | Bioinert ULA
- Is it possible to work with Matrigel™ in a µ-Slide 2 Well Co-Culture?
- Can I combine the µ-Dish 35 mm, high Bioinert with a Culture-Insert or a micro-Insert?
- I want to culture and image my spheroids on the non-adhesive Bioinert surface for a couple of days. How do I exchange the medium without aspirating the spheroids?
- How are ibidi Bioinert products stored?
- How long is the shelf life of the ibidi Bioinert surface?
- Single cells and spheroids do not attach to Bioinert. Does this mean that the spheroids can swim off the surface during imaging? If yes, how can this be avoided?
- Standard ultra-low attachments cultureware (ULA) is not suitable for fluorescence microscopy or high-resolution imaging. Is the µ-Dish 35 mm, high Bioinert suitable for these applications?
- Can I detach spheroids/organoids from an ibidi micropattern for analysis?
- How do you keep the cells in focus during a Matrigel™ assay?
- How stable is the Bioinert surface? Will touching the Bioinert surface with a pipette tip damage it?
- Bioinert is hydrogel-based. Does any swelling occur?