Cell Culture Under Flow
Biological Background
Several adherent cell types are exposed to shear stress in vivo, for example endothelial cells in a blood vessel. Culturing cells in vitro under perfusion simulates this mechanical stimulus and induces a more physiological behavior.
Course Objectives
In this course, you will learn about the theory behind flow parameters and their biological relevance. In addition to planning an experiment (including shear stress calculation, choice of equipment and possible read-outs), you will also learn how to setup and operate the ibidi Pump System, as well as handle the ibidi Channel Slides, including cell culture and immunostaining. During the two-day training, our experts will give you tips and tricks that will be helpful with your own experiments.
Target Group
The course is intended for scientists and technical associates, who have extensive experience with cell culture and sterile working techniques, and want to establish cell culture under flow experiments using the ibidi Channel Slides and the ibidi Pump System.
Course Dates
In-house practical courses will take place on demand. Please contact us at: info@ibidi.de for more information.
Check out our Online Lab Courses.
What Our Participants Say
"Actually the practical course “Cell Culture Under Flow” in Munich helped me a lot to optimize my assay, especially by taking the shear rate aspects into consideration in addition to shear stress, and playing with medium viscosity to get better compromise. Very helpful, so thanks again for the coordination that allowed us to attend!"
Laurent Klein, Novartis Pharma AG, Basel, Switzerland