µ-Slide 8 Well

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µ-Slide 8 Well

An open µ-Slide (chambered coverslip) with 8 wells for use in immunofluorescence and high-end microscopy

All-in-one chamber slide reduces the number of experimental steps in immunofluorescence protocols

Observation of the sample through the coverslip-like bottom using high resolution microscopy

Fast and easy sample preparation: No coverslips, no leakage

Cost-effective experiments using small numbers of cells and low volumes of reagents
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An open µ-Slide (chambered coverslip) with 8 wells for use in immunofluorescence and high-end microscopy

All-in-one chamber slide reduces the number of experimental steps in immunofluorescence protocols
Observation of the sample through the coverslip-like bottom using high resolution microscopy
Fast and easy sample preparation: No coverslips, no leakage
Cost-effective experiments using small numbers of cells and low volumes of reagents

More details

Surface Modification

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5	20%

Product Details

Applications:

Cultivation and microscopy of cell cultures
Transfection assays
Fluorescence microscopy of living and fixed cells
Live cell imaging over extended time periods
Suitable for DIC, when used with a DIC lid

Technical Features:

Open µ-Slide (chambered coverslip) with 8 independent wells
Excellent optical quality imaging chamber for high-end microscopy
Compatible with staining and fixation
ibiTreat surface for optimal cell adhesion
Biocompatible plastic material – no glue, no leaking
Also available in an adhesive version without a bottom: sticky-Slide 8 Well
Also available with a glass bottom or with a relocation grid

Specifications:

Number of wells	8
Dimensions of wells (w x l x h) in mm	9.4 x 10.7 x 6.8
Volume per well	300 µl
Total height with lid	8 mm
Growth area per well	1.0 cm²
Coating area per well	2.20 cm²
Bottom: ibidi Polymer Coverslip

Define and print your experimental setup

The ibidi µ-Slide 8 well allows standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (i.e., cell cultivation, fixation, staining and imaging) are carried out in the classic open well geometry. After staining, the sample can be observed through the coverslip-like bottom using high resolution microscopy. The closely fitting lid prevents evaporation and allows for long-term assays.

Closely fitting lid
Suitable for high resolution microscopy

Other Possible Applications:

Can be used with Culture-Inserts

To test different compound concentrations, e.g., in stem cell development

Supporting Material

User Comments

Madeleine Zerbato, Brigham Regenerative Medicine Center, Cambridge, USA

"The ibidi µ-Slide 4 Well and µ-Slide 8 Well that I am working with are actually the best products I have tested so far for cultivating D3 mouse embryonic cell lines. They attach well to the treated slide (ibiTreat µ-Slides), and the resulting morphology is very similar to the one they have on regular plastic. That is something very unique to those microscopic slides, since D3 mESCs do not attach to glass and form embryoid-like structures. I tested other non-glass slides from other manufacturers but the ibiTreat µ-Slides are the only ones that give me a physiological-like morphology.”

Madeleine Zerbato
Brigham Regenerative Medicine Center
Cambridge, USA

http://brmc.bwh.harvard.edu/

Jessica Davis, University College Dublin, Ireland

"I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment—I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”

Jessica Davis
University College Dublin
Ireland

http://www.ucd.ie/

Stefanie Kirchberger, Children's Cancer Research Institute, Vienna, Austria

"We tested the µ-Slide 2 Well and µ-Slide 8 Well in the uncoated version for the confocal microscopy of fish larvae. Both types of slides worked fine on our confocal microscope. We have already ordered another bunch of those slides for the lab!"

Stefanie Kirchberger
Children's Cancer Research Institute
Vienna, Austria

http://science.ccri.at/

Ivan Bedzhov, University of Cambridge, Gurdon Institute, Cambridge, United Kingdom

“We looked for an imaging-compatible material that supports embryo attachment. This led us to establish that we can successfully grow and image mouse embryos on ibiTreat μ-Plates 24 Well and µ-Slides 8 Well from ibidi, which have optical properties similar to glass, and allow efficient blastocyst attachment. Thus, ibidi slides and plates are now an indispensable part of our in vitro culture methodology.“

Ivan Bedzhov
University of Cambridge
Gurdon Institute
Cambridge
United Kingdom

http://www.gurdon.cam.ac.uk/

Owen McCarty, PhD, Oregon Health & Science University, Portland, USA

“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”

Owen McCarty, PhD
Associate Professor
Department of Biomedical Engineering
Oregon Health & Science University
Portland, Oregon
USA

http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/biomedical-engineering/

Joanne Marrison, University of York, UK

"I plated and transfected cells on the ibidi µ-Slide 8 Well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are 'slide' sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great."

Joanne Marrison
Imaging and Cytometry Laboratory (B/K051)
Bioscience Technology Facility, Department of Biology
University of York, York
United Kingdom

www.york.ac.uk/biology/tf

Prof. Dr. Markus Sauer, University of Wuerzburg, Germany

„ibidi slides are compelling!
We tested the open μ-Slides with 8 wells for live-cell super-resolution imaging by dSTORM with SNAP-tags. The quality of the super-resolved images was impressive.“

Prof. Dr. Markus Sauer
Biotechnology & Biophysics
Julius-Maximilians-University Wuerzburg
Germany

http://www.super-resolution.biozentrum.uni-wuerzburg.de/startseite/