µ-Slide 8 Well

Instructions

• Instructions µ-Slide 8 Well (PDF)

Application Notes

• AN 08: Cell Culture Coating (PDF)
• AN 16: Fluorescence Staining using a µ-Slide 8 Well (PDF)
• AN 33: Live / Dead Staining with FDA and PI (PDF)

Movies

• MV 05: Mitochondria Stain

Product Flyer

• Product Flyer ibidi μ-Dishes, μ-Slides, and μ-Plates (PDF)
• Product Flyer µ-Slide 2 Well | 4 Well | 8 Well (PDF)
• Product Flyer Immunofluorescence (PDF)

FAQ

• µ-Slides and µ-Dishes
• Which solvents and chemicals are compatible with the ibidi labware?
• Which immersion oils are compatible with ibidi products?

Lee Shu Ying, National University of Singapore, Singapore

"As a microscopy core facility, we have to recommend the most suitable imaging plates to our users for their experiments. The µ-Slide 8 Well is our best seller amongst the other wonderful platforms that ibidi carries.

We had multiple experiments where the cells/bacteria were not adhering well to glass coverslips, but grew perfectly on the ibiTreat surface. Confocal images turned out great as well. Our users also love how the cover fits snugly for ease of handling."

Lee Shu Ying
National University of Singapore
Yong Loo Lin School of Medicine
Singapore

http://nusmedicine.nus.edu.sg/medical-sciences-cluster/confocal-microscopy-unit/

Madeleine Zerbato, Brigham Regenerative Medicine Center, Cambridge, USA

"The ibidi µ-Slide 4 Well and µ-Slide 8 Well that I am working with are actually the best products I have tested so far for cultivating D3 mouse embryonic cell lines. They attach well to the treated slide (ibiTreat µ-Slides), and the resulting morphology is very similar to the one they have on regular plastic. That is something very unique to those microscopic slides, since D3 mESCs do not attach to glass and form embryoid-like structures. I tested other non-glass slides from other manufacturers but the ibiTreat µ-Slides are the only ones that give me a physiological-like morphology.”

Madeleine Zerbato
Brigham Regenerative Medicine Center
Cambridge, USA

http://brmc.bwh.harvard.edu/

Jessica Davis, University College Dublin, Ireland

"I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment—I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”

Jessica Davis
University College Dublin
Ireland

http://www.ucd.ie/

Stefanie Kirchberger, Children's Cancer Research Institute, Vienna, Austria

"We tested the µ-Slide 2 Well and µ-Slide 8 Well in the uncoated version for the confocal microscopy of fish larvae. Both types of slides worked fine on our confocal microscope. We have already ordered another bunch of those slides for the lab!"

Stefanie Kirchberger
Children's Cancer Research Institute
Vienna, Austria

http://science.ccri.at/

Ivan Bedzhov, University of Cambridge, Gurdon Institute, Cambridge, United Kingdom

“We looked for an imaging-compatible material that supports embryo attachment. This led us to establish that we can successfully grow and image mouse embryos on ibiTreat μ-Plates 24 Well and µ-Slides 8 Well from ibidi, which have optical properties similar to glass, and allow efficient blastocyst attachment. Thus, ibidi slides and plates are now an indispensable part of our in vitro culture methodology.“

Ivan Bedzhov
University of Cambridge
Gurdon Institute
Cambridge
United Kingdom

http://www.gurdon.cam.ac.uk/

Owen McCarty, PhD, Oregon Health & Science University, Portland, USA

“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”

Owen McCarty, PhD
Associate Professor
Department of Biomedical Engineering
Oregon Health & Science University
Portland, Oregon
USA

http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/biomedical-engineering/

Joanne Marrison, University of York, UK

"I plated and transfected cells on the ibidi µ-Slide 8 Well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are 'slide' sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great."

Joanne Marrison
Imaging and Cytometry Laboratory (B/K051)
Bioscience Technology Facility, Department of Biology
University of York, York
United Kingdom

www.york.ac.uk/biology/tf

Prof. Dr. Markus Sauer, University of Wuerzburg, Germany

„ibidi slides are compelling!
We tested the open μ-Slides with 8 wells for live-cell super-resolution imaging by dSTORM with SNAP-tags. The quality of the super-resolved images was impressive.“

Prof. Dr. Markus Sauer
Biotechnology & Biophysics
Julius-Maximilians-University Wuerzburg
Germany

http://www.super-resolution.biozentrum.uni-wuerzburg.de/startseite/