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µ-Dish 35 mm, high Glass Bottom

µ-Dish 35 mm, high Glass Bottom View larger

A 35 mm imaging dish with a glass bottom, suitable for use in TIRF and single molecule applications

  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass (# 1.5H)
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid
  • Also available with a relocation grid: Grid-50 / Grid-500

More details

#1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized

Product Details

Applications

  • Cultivation and microscopy of cell cultures
  • Fluorescence Correlation Spectroscopy (FCS)
  • TIRF and single molecule applications

Specifications

Ø µ-Dish 35 mm
Volume 2 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 14/12 mm
Bottom Glass coverslip No. 1.5H,
selected quality,
170 µm +/- 5 µm

Technical Features

  • Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
  • Standard format dish with a 35 mm diameter and high walls
  • May require coating to promote cell attachment

Experimental Examples


Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.


Fluorescence microscopy of fixed rat fibroblasts on a Glass Coverslip #1.5H. F-actin filaments are stained with phalloidin (green), nuclei are stained with DAPI (blue).

Detailed Comparison ibidi Dishes 35 mm

µ-Dish 35 mm, high
(Polymer Coverslip,
ibiTreat / Uncoated)

81156, 81151
Glass Bottom Dish 35 mm

81218-200
81218-800
Bottom Thickness
180 µm (+10/-5 µm)
170 µm (+/-5 µm)
170 µm (+20/-10 µm)
Bottom Material
#1.5 Polymer coverslip
(ibidi)
#1.5H glass coverslip
(D 263 M Schott high precision glass)
#1.5 glass coverslip
(D 263 M Schott high precision glass)
Lid
Lid lock for minimal evaporation
and safe handling
Lid lock for minimal evaporation
and safe handling
Standard
Surfaces
ibiTreat (tissue culture treated),
Uncoated (hydrophobic)
Uncoated glass
Uncoated glass
Gas permeable
Yes
No
No
Packaging
Sterile, single-packed
Sterile, single-packed
Sterile, packed at 10 pcs.
Quantity
60 pcs./box
60 pcs./box
200/800 pcs./box
Free Sample
Yes
Yes
Yes

Microscopy Applications

High resolution microscopy1
Confocal
+
+
+
Brightfield, Phase Contrast, DIC
+
+
+
Widefield Fluorescence
+
+
+
TIRF
-
+
-
Super-Resolution microscopy2
STED, STORM, etc.
-
+
-

Optical Properties

Refractive Index
(nD 589 nm)
1.52
1.52
1.52
Abbe number
55
56
56
Autofluorescence
Low
Low
Low
Transmission
Very high (even ultraviolet)
High (ultraviolet restrictions)
High (ultraviolet restrictions)
Birefringence (DIC)

Note:

1 High resolution microscopy = high magnification (40x - 100x) and high resolution microscopy including widefield or confocal techniques (e.g., brightfield, DIC, fluorescence). The resolution is limited to ~200 nm by the diffraction limit. Does not include Super-Resolution.
Resolution is described as a function to discriminate 2 dots from each other; it is dependent on wavelength and numerical aperture. It is physically limited by Abbe’s law which can only be overcome by Super-Resolution microscopy.

2 Super-Resolution microscopy = additional resolution by overcoming the diffraction limit with 63x - 100x oil immersion objectives. Super-Resolution provides a resolution below 200 nm, down to 5-20nm by computational (PALM, SIM, STORM, RESOLFT) and one optical Super-Resolution technology (STED). TIRF is not a Super-Resolution technology.

³ DIC Compatibility = The lids are not DIC compatible. Special glass DIC Lids for the µ-Dishes are available separately.

Supporting Material

User Comments

Contu Raluca, National Center of Neurology and Psychiatry, Tokyo, Japan

"We often use ibidi glass bottom dishes for live imaging of cultured cells. We were able to get very good images of protein delocalization, as well as of nucleic acids taken up by living cells. The optical quality of the bottom of the dishes is extraordinary. Actually, two of our papers for which we used ibidi µ-Dishes and μ-Slides were published in 2017:

Contu et al. 2017 J Cell Sci. http://jcs.biologists.org/content/130/17/2843.long

Takahashi et al. 2017 RNA Biology http://www.tandfonline.com/doi/full/10.1080/15476286.2017.1302641

Contu Raluca
National Center of Neurology and Psychiatry
Tokyo
Japan

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