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DIC Lid for µ-Dishes

DIC Lid for µ-Dishes View larger

Optimized for inverted microscopy

Special lid with a glass insert for use in Differential Interference Contrast (DIC) microscopy

More details

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Product Details

Applications

Specifications

Compatible with ibidi µ-Dishes 35 mm, high
ibidi µ-Dishes 35 mm, low
Material Polymer, glass coverslip window

Technical Features

  • Lid with glass coverslip insert
  • Low birefringence for excellent DIC imaging
  • Compatible with all ibidi 35 mm µ-Dishes
  • Not compatible with the Glass Bottom Dish 35 mm

The DIC Beam Path

For excellent imaging with a differential interference contrast (DIC) microscope, all elements in the optical pathway need to be DIC-compatible. They must exhibit a low birefringence, meaning that there is only low interference with the polarized light. Just one single incompatible element in the beam path of the transmitted light would destroy the orientation of the polarized light, leading to disturbance of the DIC imaging effect. When using the ibidi µ-Dishes combined with the special DIC Lids for µ-Dishes , excellent DIC images can be generated.

DIC Compatibility of Different Materials

Compatible

Not compatible
  • Lids of all µ-Slides, µ-Dishes and µ-Plates
  • Channel µ-Slides and Ph+ µ-Slides
  • Cell culture flasks, Petri dishes

Experimental Example

Human keratinocytes on an ibiTreat surface. Imaging with a DIC objective lens 10x.

Supporting Material

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