Coatings: Collagen I, Collagen IV and Poly-L-Lysine

For selected µ-Slides, ibidi offers Collagen I, Collagen IV and Poly-L-Lysine precoated surfaces on the ibidi Polymer Coverslip. We also provide a high-quality Collagen Type I solution for preparing 3D gels and surface coatings.

In the ibidi Application Note Cell Culture Coating (AN 08) (PDF), you will find detailed information on how to do your own coating on µ-Slides using collagen I, collagen IV, fibronectin, poly-L-lysine, or poly-D-lysine.

Please contact us for more coating options.

RAT1 cells on the ibidi Polymer Coverslip coated with collagen IV.

Collagen I Coating

Collagen type I is the most common type of collagen in the human body. Building collagen fibers, it is present in many parts of the body, such as skin and bone. To promote better cell adherence, culture vessel surfaces, such as the ibidi Polymer Coverslip, can be coated with collagen I. In addition, collagen I 3D gels are frequently used for cell cultivation to stimulate an in vivo-like 3D environment.

ibidi provides ready-to-use µ-Slides that are coated with Collagen Type I, Rat Tail (ibidi, 50203). For researchers who want to do their own coating, ibidi offers a high-quality Collagen Type I that is made from either bovine dermis or rat tail tendon.

Collagen IV Coating

Collagen type IV is one of the major constituents of the basement membrane, which is a thin layer of the extracellular matrix (ECM). In cell culture, collagen IV is used as a substrate to promote the adherence and proliferation of cells. Collagen IV coatings have been tested for a variety of standard cell lines, such as epithelial, endothelial, neural, and muscle cells. ibidi uses mouse collagen IV (Corning, No. 356233) for the coating.

Poly-L-Lysine (PLL) Coating

Poly-L-lysine (PLL) is a polymer of the essential amino acid L-lysine. This polymer is one of the most commonly used adhesion substrates for cell culture. It is suitable for a large variety of cell types, especially for neuronal cultures. Adhesion is mediated by an integrin-independent mechanism when PLL is used. ibidi uses a commercially available PLL solution (Sigma-Aldrich, No. P4832) for the coating.