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Culture-Insert 4 Well in µ-Dish 35 mm, high

Culture-Insert 4 Well in µ-Dish 35 mm, high View larger

4 well silicone insert with 4 defined cell-free gaps, suitable for wound healing, migration assays, 2D invasion assays, and co-cultivation of cells

  • Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
  • Reproducible experiments owing to: four defined 500 µm cell free gaps and one 1000 µm center gap, no leakage during cultivation, and no material being left behind after the insert’s removal
  • Suitable for 4 experiments, including a center gap that allows to observe the migration of all cell types
  • Individual inserts for single experiments
  • Ideal cell growth on ibiTreat surface

More details

ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
30
in µ-Dish 35 mm, high

Product Details

Specifications

Number of wells 4
Outer dimensions Ø 17 mm
Volume per well 110 µl
Growth area per well 0.35 cm²
Coating area per well 1.23 cm²
Width of cell free gap
- Two cell fronts 500 µm +/- 100 µm
- Four cell fronts (center) 1000 µm +/- 100 µm
Material Biocompatible silicone
Bottom No bottom - sticky underside

Technical Features

  • Culture-Insert 4 Well, pre-inserted into the µ-Dish 35 mm, high
  • Biocompatible silicone material with adhesive underside
  • Ready-to-use: the Culture-Insert is already placed in the µ-Dish 35 mm, high
  • Clean surface after insert removal without remaining material
  • Find full specifications and technical details of the µ-Dish here

Principle for Wound Healing and Migration Assays

Various Applications

Wound Healing / Migration

The Culture-Insert 4 Well allows for wound healing and migration assays with four cell-free gaps with a 0.5 mm gap width, and one center area with a 1 mm diagonal distance. Compared to the 2 well version, more cells can be analyzed and a higher throughput is realized. Two cell-free gaps create a technical replicate in the very same well.

Two Conditions of Wound Healing / Migration

With the Culture-Insert 4 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).

Four Conditions for Co-Cultivation / Invasion

By seeding up to four different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.

Invasion and co-cultivated cells can be imaged by using high-resolution fluorescence microscopy.

Visualization of Cell Migration

Live cell imaging of a wound healing and migration assay using the MCF7 cell line. Objective lens 10x, phase contrast microscopy.

Supporting Material

Movies

User Comments

Ali Alhoshani, King Saud University, Riyadh, Saudi Arabia

“The ibidi Culture-Insert 2 Well 24 plate was unbelievably helpful when I wanted precise and consistent results for wound healing assay. It saved my lab so much time and effort. The web-based quantitative image analysis feature is a huge plus. Thank you for such useful products. "

Ali Alhoshani
King Saud University
Department of Pharmacology and Toxicology
Riyadh
Saudi Arabia

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