Cytoskeletal dynamics: - Actin dynamics in cellular processes - Live cell imagingand microscopy
Superb biocompatibility - non-toxic staining of living samples
Up to 100% transduction efficiency
NOTE: Please note that you will be working with a product containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL–2 organisms.
Check out the ibidi Adenovirus Transduction Enhancer ibiBoost for fast and efficient adenovirus transduction.
1 x 1010 IU / ml
TagGFP2 (Exmax / Emmax)
483 / 506 nm
TagRFP (Exmax / Emmax)
555 / 584 nm
LifeAct, a 17-amino acid peptide, is derived from a protein from Saccharomyces cerevisiae. LifeAct stains filamentous actin (F-actin) structures in living or fixed eukaryotic cells and tissues. In contrast to GFP-actin and its alternatives such as actin-binding proteins, LifeAct does not interfere with actin dynamics in vitro and in vivo, as shown in several cell types and model organisms.*
LifeAct-Adenoviral Vectors are used for difficult-to-transfect cells or when up to 100 % transgene expressing cells are needed. For many cell lines, the CMV (cytomegalovirus) promoter is the best choice as it leads to strong expression of LifeAct.
After the transduction of cells with rAV-LifeAct, F-actin is visualized using the fluorescence markers TagGFP2 or TagRFP**. With this method, transduction efficiencies up to 100% can be attained, even in difficult-to-transfect cell types like primary cells (e.g., neuronal cells). This virus can be used for transient protein expression. To generate stable cell lines, please refer to our LifeAct Lentivirus products.
LifeAct plasmids may be used in many cell types when a transient or stable expression of LifeAct is needed. In this case, it is also possible to choose between the two promoters mentioned before.
In addition, different stable cell lines are available, which help facilitate daily lab life. You can immediately start performing experiments without the time-consuming and laborious generation of stable cell lines, while still assuring high-quality standards.
Method Comparison: Using LifeAct for F-Actin Visualization in Living Cells