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µ-Slide 4 Well Glass Bottom

µ-Slide 4 Well Glass Bottom View larger

Open µ-Slide with 4 wells with a glass bottom, suitable for use in TIRF and single molecule applications

  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
  • Cost-effective experiments using small numbers of cells and low volumes of reagents

More details

#1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized

Product Details


  • Cultivation and microscopy of cell cultures
  • TIRF and single molecule applications


Outer dimensions in mm (w x l) 25.5 x 75.5
Number of wells 4
Dimensions of wells
(w x l x h) in mm
21.6 x 11.4 x 9.3
Volume per well 700 µl
Total height with lid 10.8 mm
Growth area per well 2.5 cm²
Coating area per well 4.1 cm²
Bottom Glass coverslip No. 1.5H,
selected quality, 170 µm +/- 5 µm

Technical Features

  • Open µ-Slide with 4 independent wells
  • Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
  • May require coating to promote cell attachment
  • Also available in a Ph+ version: intermediate plate in each well to avoid meniscus formation
  • Also available as a µ-Slide 4 Well with an ibidi Polymer Coverslip Bottom for superior cell growth

Experimental Example

Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatings Compatible Compatible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Applications Fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately

Supporting Material

User Comments

Serene Gwee, National University of Singapore, Singapore

"I started using the Glass Bottom chambered slides with my time-lapse imaging of zebrafish embryos and they have been very useful and applicable for my experiments."

Sze Ling Gwee
National University of Singapore
Department of Biological Sciences

Rachid Rezgui, New York University Abu Dhabi, Abu Dhabi, UAE

"We have successfully tested the µ-Slide 4 Well and 8 Well Glass Bottom. We are very satisfied with these slides. First of all, the fast delivery allows us to be more flexible when we plan our experiments. We tried Fluorescence correlation spectroscopy to study the interaction of Cell Penetrating Peptides with small interfering DNA and obtained great results. As FCS measures the concentration in the focal volume, it is extremely sensitive to surface changes. As we used glass bottom dishes, we were able to achieve a very small focal volume for maximum precision. We also used them for confocal imaging on Mouse Embryonic Fibroblasts (GSK3-KO MEF) to study molecular motors and obtained great images on a Leica SP8 with Hyvolution."

Rachid Rezgui
New York University AbuDhabi
Abu Dhabi

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