µ-Slide 4 Well Glass Bottom

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µ-Slide 4 Well Glass Bottom

Open µ-Slide with 4 wells with a glass bottom, suitable for use in TIRF and single molecule applications

Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass

Cost-effective experiments using small numbers of cells and low volumes of reagents
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Open µ-Slide with 4 wells with a glass bottom, suitable for use in TIRF and single molecule applications

Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
Cost-effective experiments using small numbers of cells and low volumes of reagents

More details

Surface Modification

#1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized

Pcs./Box

Product Details

Applications

Cultivation and microscopy of cell cultures
TIRF and single molecule applications

Specifications

Outer dimensions in mm (w x l)	25.5 x 75.5
Number of wells	4
Dimensions of wells (w x l x h) in mm	21.6 x 11.4 x 9.3
Volume per well	700 µl
Total height with lid	10.8 mm
Growth area per well	2.5 cm²
Coating area per well	4.1 cm²
Bottom	Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Features

Open µ-Slide with 4 independent wells
Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
May require coating to promote cell attachment
Also available in a Ph+ version: intermediate plate in each well to avoid meniscus formation
Also available as a µ-Slide 4 Well with an ibidi Polymer Coverslip Bottom for superior cell growth

Experimental Example

Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.

ibidi Polymer Coverslip vs. ibidi Glass Bottom

	ibidi Polymer Coverslip	ibidi Glass Bottom
Optical properties
Refractive index (n_D 589 nm)	1.52	1.52
Abbe number	56	55
Thickness	#1.5 (180 µm)	#1.5H (170 µm)
Material	Microscopy plastic	D 263M Schott borosilicate glass
Autofluorescence	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)
Birefringence (DIC)	Low (DIC compatible*)	Low (DIC compatible*)

Other aspects
Surface modifications	ibiTreat – tissue culture treated Uncoated – hydrophobic	Only glass
Protein coatings	Compatible	Compatible
Gas permeable	Yes	No
Material flexibility	High	Low
Breakable	No	Yes
Applications	Fluorescence microscopy	TIRF and single photon

* Special glass DIC Lids are available separately

Supporting Material

Instructions

Instructions µ-Slide 4 Well Glass Bottom (PDF)

Application Notes

AN 08: Cell Culture Coating (PDF)

FAQ

User Comments

Serene Gwee, National University of Singapore, Singapore

"I started using the Glass Bottom chambered slides with my time-lapse imaging of zebrafish embryos and they have been very useful and applicable for my experiments."

Sze Ling Gwee
National University of Singapore
Department of Biological Sciences
Singapore

http://www.dbs.nus.edu.sg/

Rachid Rezgui, New York University Abu Dhabi, Abu Dhabi, UAE

"We have successfully tested the µ-Slide 4 Well and 8 Well Glass Bottom. We are very satisfied with these slides. First of all, the fast delivery allows us to be more flexible when we plan our experiments. We tried Fluorescence correlation spectroscopy to study the interaction of Cell Penetrating Peptides with small interfering DNA and obtained great results. As FCS measures the concentration in the focal volume, it is extremely sensitive to surface changes. As we used glass bottom dishes, we were able to achieve a very small focal volume for maximum precision. We also used them for confocal imaging on Mouse Embryonic Fibroblasts (GSK3-KO MEF) to study molecular motors and obtained great images on a Leica SP8 with Hyvolution."

Rachid Rezgui
New York University AbuDhabi
Abu Dhabi
UAE
www.nyuad.nyu.edu