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µ-Dish 35 mm, high ESS

µ-Dish 35 mm, high ESS View larger

A 35 mm imaging dish with an Elastically Supported Surface (ESS) used for growing cells under “in vivo-near” conditions

More details

Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized

Product Details

Applications

Specifications

Ø µ-Dish 35 mm
Volume 2 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 14/12 mm
Bottom Elastic surface (40 µm) on
glass coverslip (100 µm)

Technical Features

  • 40 μm thick PDMS (Polydimethylsiloxane) layer on top of a 100 μm thick glass cover
  • Available stiffness: 1.5 kPa, 15 kPa, and 28 kPa
  • Biocompatible soft cell culture material
  • High walls with a standard height for easy handling
  • Lid with lock position, which minimizes evaporation
  • Rim for easy opening
  • No autofluorescence
  • Fully compatible with the ibidi Heating System



For a detailed explanation on the ESS technology please refer to the Special Surfaces section of our website.

Experimental Example

Differentiated mouse fibroblasts cultured on elastic surface (ESS 28 kPa)
green: zyxin (Alexa 488 conjugated antibody)
red: alpha- smooth muscle actin (Cy5 conjugated antibody)

(courtesy R. Merkel, Jülich, Germany)

Supporting Material

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