For pricing and online shopping, please log in.

Product filter

Top sellers

µ-Dish 35 mm, low

µ-Dish 35 mm, low View larger

A 35 mm imaging dish with an ibidi Standard Bottom and low walls, used in high end microscopy and micromanipulation

  • Large access for micromanipulation
  • Unique ibidi Polymer Coverslip combining
    • Excellent cell culture conditions; and
    • Supreme optical quality for high resolution microscopy
  • Ideal cell growth conditions provided by the ibiTreat surface
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid
  • Also available with a grid

More details


Product Details


  • Cell manipulation and microinjection
  • Fluorescence microscopy of both living and fixed cells
  • Live cell imaging
  • Suitable for DIC, when used with the special DIC Lid


Ø µ-Dish 35 mm
Volume 800 µl
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 9 / 7 mm
Bottom: ibidi Polymer Coverslip

Technical Features

  • Standard format dish with a 35 mm diameter
  • Made of biocompatible material using no glues, consequently no cells are harmed
  • No autofluorescence
  • Compatible with all staining and fixation solvents

Low walls

Experimental Example

Microinjection of cells using a µ-Dish 35 mm, low. A glass microinjection capillary is connected to a micromanipulator directly on the stage of a confocal fluorescence microscope. The low walls of the µ-Dish 35 mm, low allow for easy access of the cells with the capillary.

Live cell imaging of Dictyostelium discoideum (DdLimE-GFP mutant) in the µ-Dish 35 mm, low. The image is an overlay of brightfield and fluorescence microscopy showing the recruitment of the LIM domain-containing protein E towards the pseudopodia. Objective lens 20x/0.5, Zeiss LSM 510, scale bar = 20 µm.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide, or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

ibidi µ-Dish Family: Versions and Variations

High walls:
Used for all standard applications

Low walls:
Provide greater cell access (useful for micromanipulation)

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatings Compatible Compatible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Applications Fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately

Supporting Material

19 Related Products