How much shear stress is suitable for rolling and adhesion assays?

In rolling and adhesion assays, the same parameters as for a standard flow conditioning of an endothelial cell layer should be used. There are two things to consider: Firstly, the endothelial cell layer should be flow-conditioned for several days before performing a rolling and adhesion experiment. The shear stress for this period might be different from the shear stress applied during the rolling and adhesion. Secondly, in adhesion assays, the speed of the perfused medium also plays a role. A variation of the shear stress might be needed. Also, it is necessary to test the optimal flow rate/shear stress.

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