Does the Heated Lid of the ibidi Stage Top Incubators affect the microscopic resolution?

No, the Heated Lid does not affect the microscopic resolution. When working with transmitted light there is no reported effect on the optical path, because the window in the Heated Lid of the ibidi Stage Top Incubators is made of glass. Thus, the resolution remains unchanged. In theory, there is a small percentage of intensity loss during light transmission, but this is not an issue for transmitted light.

When working with fluorescence on an inverted microscope, the optical path does not pass the Heated Lid, since both the light for excitation of the sample and the fluorescent light emitted from the sample only need to pass the space between the objective and the sample. Thus the glass of the Heated Lid does not influence the resolution.

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