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Collagen Type I, rat tail
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Note: If you want to prepare 3D gels either with or without cells, we recommend using the 5 mg/ml Collagen I solution. We do not recommend using the 10 mg/ml Collagen solution for these applications.
Chemotaxis of HT-1080 cells, which are migrating towards FCS, using low resolution microscopy for a maximum of migration data
High resolution microscopy during chemotaxis and cell polarization, using the HT-1080 LifeAct cell line
Cell spheroid (or micro tumor) on top of, or embedded in, a 3D matrix for long-term cultivation. The upper well provides nutrients by means of diffusion.
Instructions Collagen Type I, rat tail, 5 mg/ml (PDF) Instructions Collagen Type I, rat tail, 10 mg/ml (PDF)
Material Safety Data Sheet Collagen Type I (PDF)
AN 26: Collagen I Gel for 3D Cell Culture AN 17: Chemotaxis 2D and 3D (PDF) AN 08: Cell Culture Coating (PDF) AN 24: Chemotaxis of HT-1080 Cells in 2D and 3D AN 34: Chemotaxis of HUVEC Cells in 2D and 3D
MV 25: ibidi Chemotaxis Assays
Qualitative and quantitative analysis of slowly migrating chemotactic cancer cells in 3D (PDF)
How do I prepare a 3D gel using the collagen solution that you provide?My gel looks very inhomogeneous. What is the reason for that?
ibidi Chemotaxis Assays (MV 25)
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