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Collagen Type I, rat tail

Collagen Type I, rat tail View larger

A high quality rat tail collagen solution used for creating 3D collagen gels (e.g., in chemotaxis assays)

  • Provides in vivo–like ECM (extracellular matrix) structures
  • High concentrated collagen solution – usable for a variety of gel concentrations
  • Fast polymerizing for optimal cell distribution in gel
  • Non-pepsinized, native collagen for a close-to-nature situation

Product Details

Applications:

  • Chemotaxis assays in firm 3D collagen gels
  • Leukocyte and tumor cell migration
  • 3D cell microscopy
  • Culturing cells on thin or thick collagen layers
  • Coating of cell cultureware to promote cell attachment
  • Differentiation studies – 2D versus 3D
  • Highly suited for live cell imaging and fluorescence microscopy

Technical Features:

  • High-quality purified protein from rat tendons
  • Ideal for use in combination with µ-Slide Chemotaxis
  • Ready to use
  • Highly standardized manufacturing process
  • Stringent quality standards
  • Simple and easy protocol and handling
  • Storage at -20°C for well-defined quality and reproducibility

Specifications:

Source Rat tail tendon
Appearance Optically clear viscous liquid
Extraction Acid, non-pepsinized
Purity > 90 % by SDS PAGE
Sterility Sterile, for cell culture
Contaminants Negative for DNA, bacteria, fungi, and mycoplasma
Growth factors None
Formulation Supplied in 17.5 mM acetic acid (0.1%)
pH pH 3.8
Functional control 3D gelling and 2D coating test in cell culture
Storage -20°C


Note: If you want to prepare 3D gels either with or without cells, we recommend using the 5 mg/ml Collagen I solution. We do not recommend using the 10 mg/ml Collagen solution for these applications.

Application Examples

Chemotaxis of HT-1080 cells,
which are migrating towards FCS,
using low resolution microscopy
for a maximum of migration data

High resolution microscopy during chemotaxis and cell polarization, using the HT-1080 LifeAct cell line

Cell spheroid (or micro tumor) on top of, or embedded in, a 3D matrix for long-term cultivation. The upper well provides nutrients by means of diffusion.

Supporting Material

Movies

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