1 High resolution microscopy = high magnification (40x - 100x) and high resolution microscopy including widefield or confocal techniques (e.g., brightfield, DIC, fluorescence). The resolution is limited to ~200 nm by the diffraction limit. Does not include Super-Resolution. Resolution is described as a function to discriminate 2 dots from each other; it is dependent on wavelength and numerical aperture. It is physically limited by Abbe’s law which can only be overcome by Super-Resolution microscopy.
2 Super-Resolution microscopy = additional resolution by overcoming the diffraction limit with 63x - 100x oil immersion objectives. Super-Resolution provides a resolution below 200 nm, down to 5-20nm by computational (PALM, SIM, STORM, RESOLFT) and one optical Super-Resolution technology (STED). TIRF is not a Super-Resolution technology.
³ DIC Compatibility = The lids are not DIC compatible. Special glass DIC Lids for the µ-Dishes are available separately. Please note: the Glass Bottom Dish 35 mm is not compatible with the DIC Lids for µ-Dishes.
"I tested the dishes with glass bottom for fluorescence microscopy applications. We used U20S cells, plated on the ibidi Glass Bottom Dish 35 mm, and performed time-lapse imaging. I found the quality of the samples very good. We could get sharp microscopy pictures."
Francesca Sartor Ludwig-Maximilians-University München Germany