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ibidi Heating System, Universal Fit, for 1 Chamber

ibidi Heating System, Universal Fit, for 1 Chamber View larger

Heating System for all live cell imaging applications including high resolution, TIRF, and confocal microscopy

  • Stage top incubator that is compatible with all microscopy platforms - fits every inverted microscope that has a regular frame or holder for 96 well plates
  • Excellent illumination of the sample - no condensation due to heated lid, optimal magnetic sample stabilization, and suitable for DIC
  • Perfectly suited for live cell imaging applications - full incubator conditions on the microscope
  • Available with or without Heated Lid

System includes:

ibidi Temperature Controller

Heated Plate for 1 Chamber in Multi-Well Format

Heated Glass Lid, Universal Fit

Heating Insert for ibidi
and non-ibidi Slide Formats

Product Details


Rat fibroblasts (cell line)


Temperature Controller
Operating humidity Max. 80% relative humidity (RH)
Environmental operating temperature 18–30°C/64–86°F (min 5°C/9°F less than set temperature)
Dimensions 90 mm x 170 mm x 230 mm
Weight 1720 g / 3.8 lbs
External power supply AC 100–240 V, 50/60 Hz, 2 A
Heated Plate and Lid
Plate dimensions 127.5 x 85.5 x 12 mm³
(fits standard multiwell holders)
Lid dimensions 127.5 x 85.5 x 19 mm³
Height of the system 25.5 mm
Weight of incubation chamber
with insert
330 g / 0.46 lbs
Gas inlet Luer connector with Luer Lock
Restrictions for objective lenses None
Custom openings On request

Heating Stages for Live Cell Imaging - No Condensation

The independently controlled, heated glass lid of the ibidi Heating & Incubation System solves the problem of condensation in live cell imaging.

By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.

Components of the ibidi Heating & Incubation System, Universal Fit

ibidi Temperature Controller

The Temperature Controller controls the temperature of the different heated components (Heated Lid and Heated Plate). Four control channels are available.

Technical Features:

  • Temperature stability: ± 0.05 °C
  • Temperature uniformity across the insert: ± 0.5 °C
  • Temperature range: Room temperature - 45 °C
  • Control channels: 4
  • IncuControl software for remote control and data recording included
  • External temperature sensor included
  • Adjustable alarm function included
  • Analog output for extreme noise reduction optional

Heated Plate in a Multi-well Format for 1 Chamber

  • Can be used with any inverted microscopy system
  • Fits into both manual and automated stages
  • Can be placed into any regular multi-well frame that comes with your microscope
  • Adaptable to all types of microscopy chambers, such as µ-Slides and µ-Dishes plus non-ibidi formats

Heated Plate in a Multi-well Format for 4 µ-Slides

  • Can be used with any inverted microscopy system
  • Ideal for use with motorized stages
  • Can be placed into any regular multi-well frame that comes with your microscope
  • Compatibe with all ibidi µ-Slides and non-ibidi slide formats

Heated Glass Lid, Universal Fit, for Use with CO2 and Humidity

  • For use with CO2, O2, and humidity to provide a complete environmental chamber
  • Independently controlled glass lid, warmed to 40 °C, prevents the formation of condensed water on the lid of the microscopy chamber or plate
  • No scattering of light or shadow formation

Heating Inserts

Optional: Heating Insert Adapter for Perfusion Assays

Gas Mixer for the Incubation Unit

The heated lid also comes with a gas inlet. The ibidi Gas Incubation System upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications, including high resolution, TIRF, and confocal microscopy.

The ibidi Gas Incubation System is suitable for various experimental conditions (e.g., pH or hypoxia). It is available in two versions: Gas Mixer for CO2, Gas Mixer for CO2 and O2.

Live Cell Imaging Under Flow Conditions

The ibidi Heating & Incubation System can be adapted for live cell imaging under flow conditions. Connect the ibidi Pump System with the ibidi Heating System, Universal Fit, by using the Heating Insert Adapter for perfusion assays. This creates the perfect environment for long-term cell studies (several days) under flow conditions.

Overview of the Heating and Incubation System, Universal Fit

ibidi Heating System for Temperature Control of Multi-Well Plates

ibidi has developed a Heating System for the use with K-Frame Stages or the Nikon Ti-S-E and Ti-S-ER Motorized Stage which hold multi-well plates. Thus, this system permits the user to control the temperature of samples held in multi-well plates.

Special Solutions

You can also obtain additional customer-specific solutions upon request.

Supporting Material

User Comments

Tim Sanchez, Harvard University, Cambridge, USA

"For my research, I need to take time-lapse images of mouse embryos developing from the 1 cell stage all the way to 5-day blastocyst. Anyone who has worked with mouse embryos knows they are incredibly sensitive and delicate, so we required an extremely stable environment over several days. I struggled for months with a different system, but the ibidi Heating Stage worked very quickly. I was immediately impressed with the quality. Calibration with the included thermocouple was easy and accurate. Now I get consistent development every time, and the percentage of embryos developing to blastocysts is the same as the control incubator! Terrific product, thanks ibidi.”

Tim Sanchez, Harvard University, Cambridge, USA

Loïc Dupré, INSERM, Toulouse, France

"The ibidi µ-Slides Chemotaxis 2D and 3D have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.

I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"

Loïc Dupré PhD, INSERM UMR 1043, Purpan University Hospital, Toulouse, France

Prof. Dr. Stefan Zahler, University of Munich, Germany

„Using the „angiogenesis slide“ from ibidi is the only possibility that I know for achieving a consistently good optical quality in the tube formation assay, and saving Matrigel at the same time.

In comparison to other systems, the heating stage from ibidi shows superior thermal stability and enables you to work with high humidity (>80%). This is not possible with other systems but is indispensable for long-term studies.“

Prof. Dr. Stefan Zahler, Pharmaceutical Biology, Munich Center for System-Based Drug Research, Ludwig-Maximilians-University, Germany

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