The ibidi Perfusion System Perfectly Reflects the Natural Environment of Cells Under Flow Conditions
The ibidi Pump System consists of two main components: The ibidi Pump (computer controlled air pump, placed outside the incubator) and the Fluidic Unit (holder for cell media reservoirs and μ-Slide, tubing, and electrically controlled valve set, placed inside the incubator). By using this “split” approach, the closed circulation of the medium can be assembled separately in the laminar flow hood and transferred to the microscope or the incubator, without compromising the sterility of the system. The open architecture using the Luer connectors allows the use of any kind of flow devices. The PumpControl software controls the pressure, and subsequently the shear stress acting on the cells, and provides the possibility to program complex flow patterns.
Placing the Fluidic Unit inside a large-size microscope incubator allows for running long-term perfusion assays directly on the microscope. A defined temperature and CO2 concentration is assured over the whole perfusion, which is ncecesary for avoiding air bubbles. For short-term experiments, the usage of a small incubator, hosting the Fluidic Unit, next to the microscope is sufficient.
Principle of Unidirectional Flow
Applying pressured air to the reservoirs of the Fluidic Unit moves the medium and generates the flow in the μ-Slide channels. In order to avoid wasting any medium, the liquid is pumped back and forth between reservoir A and reservoir B by switching the valve V1.
To assure a unidirectional flow in the channel, the other valve V2 is switched simultaneously. As the tubing parts inserted in the valve V2 are criss-crossed, the medium is always directed to the same end of the channel slide.
"Thank you for the great products that you have developed over the years. The ibidi Pump System has immeasurably helped our research. We have recently published a paper in “Stem Cells” that uses your flow system, theµ-Slide I Luer, and the µ-Plate 96 Well very effectively. It seems to me that ibidi are constantly coming up with clever and useful products, and I like to keep an eye on what interesting things are coming out next from them."
Shane R. McSweeney, Cardiovascular Division, BHF Centre of Research Excellence, School of Medicine, King’s College London, United Kingdom
"Theµ-Slide I Luer facilitates the culture of endothelial cells under defined complex flow patterns. We are particularly interested in the effects of oscillatory shear stress on redox signaling pathways, and the ibidi Air Pump and Fluidic Units are ideal systems for this purpose. The µ-Slides are convenient for both live and fixed cell imaging as well as the collection of protein and RNA samples and other cell analyses. The system has allowed us to easily investigate physiological shear patterns in vitro without a background in engineering.”
Shane R. McSweeney Cardiovascular Division BHF Centre of Research Excellence School of Medicine King’s College London United Kingdom
Dr. Nynke van den Akker, Maastricht University, Maastricht, The Netherlands
“We've been working with the ibidi Pump System and Labware for over 5 years now and have recommended it to numerous colleagues as well. The plastics are ideal for cell culture and all forms of imaging, including automated imaging (easy to focus) and all sorts of fluorescence (clear, optically superb plastics). The ibidi Pump System in fact made the endothelial cell under flow the default of our lab! And above all, the technical support is always fast and efficient.”
Dr. Nynke van den Akker Department of Cardiology Maastricht University Maastricht The Netherlands