Fuse-It-mRNA vesicles were filled with mRNA LifeAct-TagGFP2 and fused with human iPSC-derived cardiomyocytes. 16 hours after mRNA transfer, the contractions per minute were measured. The myocytes showed contraction rates of about 70 beats per minute. This value is in the normal range for unmodified myocytes which show 50 to 80 contractions per minute.
mRNA LifeAct®-TagGFP2 is a mammalian mRNA that encodes the LifeAct-TagGFP2 fusion protein. LifeAct, a 17-amino acid peptide, is derived from a protein found in Saccharomyces cerevisiae. It stains filamentous actin (F-actin) structures in living or fixed eukaryotic cells and tissues with excellent signal-to-noise ratio [Riedl et al., 2008]. mRNA LifeAct-TagGFP2 can be used for the fluorescent labeling of the actin cytoskeleton in various living cell types. For more information on the TagGFP2 reporter, please visit evrogen.com. The vector backbone also contains an ARCA cap, a strong Kozak sequence, and a poly(A) tail for efficient protein expression.