For pricing and online shopping, please log in.

Product filter

Top sellers

LifeAct-TagGFP2 Protein

LifeAct-TagGFP2 Protein View larger

A recombinant protein for fast staining and immediate analysis of F-actin in living and fixed cells

  • Brilliant visualization of F-actin for the investigation of cytoskeletal organization and cellular dynamics
  • Fastest staining of F-actin in living cells—get your results within minutes
  • Non-toxic alternative to phalloidin for the staining of living and fixed cells
  • Unrestricted actin functionality—no interference with cytoskeletal dynamics

Product Details

Applications:

Technical Features:

  • Recombinant protein, lyophilized
  • C-terminal His-tag
  • Established protocols for F-actin staining in living and fixed cells
  • Excellent signal-to-noise ratio
  • Non-toxic F-actin staining of living samples with superb biocompatibility

Specifications:

Form Lyophilized
Storage -80 °C
TagGFP2 (Exmax / Emmax) 483 / 506 nm


LifeAct, a 17-amino acid peptide, is derived from a Saccharomyces cerevisiae protein. LifeAct stains filamentous actin (F-actin) structures in living or fixed eukaryotic cells. In contrast to GFP-actin and its alternatives (e.g., actin-binding proteins), LifeAct does not interfere with actin dynamics in vitro and in vivo, as shown in several cell types and model organisms. *

F-actin Visualization in Living Cells Using LifeAct-TagGFP2 Protein

The LifeAct-TagGFP2 Protein is perfectly suited for the quick and efficient visualization of the cytoskeleton in living cells. For the staining procedure, you can use any method for protein transfer that works for your cells of interest.


Note:
Please keep in mind that the actual amount of protein within the cell after protein transfection is generally lower when compared to mRNA or DNA transfection. To adequately visualize intracellular F-actin, you should use a high-quality microscopy equipment.

Live cell imaging of F-actin in Rat1 fibroblasts after LifeAct-TagGFP2 Protein transfer (30 µg/ml, 3 minutes).

F-actin Visualization in Fixed Cells Using LifeAct-TagGFP2 Protein

In addition to working in living cells, the LifeAct-TagGFP2 Protein is also a fast and easy solution for efficient F-actin staining in a variety of fixed cells.

CHO-K1

HT1080

MCF7

Rat1

Formalin-fixed cells were permeabilized with Triton X-100 and incubated in LifeAct-TagGFP2 Protein solution (10 µg/ml in PBS) for 1 hour.


Note: Methanol and acetone can disrupt actin during the fixation process. Therefore, it is recommended to avoid using any methanol- or acetone-containing fixatives in combination with the LifeAct-TagGFP2 protein.

Method Comparison: Using LifeAct for F-Actin Visualization in Living Cells

Method Suitable for 1
Cell lines
+++
+++
+++
+++
+++
Primary cells
+
+++
++
+++
+++
Non-mammalian cells
-
+
+
-
-
Transfection Stability
Transient
Yes
Yes
Yes
Yes
No
Stable
Yes
No
No
No
Yes
Protocol and Analysis
Protocol simplicity
+++
++
++
+++
+++
LifeAct readout start
1–2 days
few hours
few minutes
1–2 days
1–2 days
LIfeAct readout duration
several days
(infinity if stable)
several days
up to 1 day
several days
several days
(infinity if stable)
Signal intensity
+++
+++
+
+++
+++
Extra Benefits
Prevention of overexpression
artefacts
-
+
+++
+
+
Prevention of viral response
+++
+++
+++
-
-
Biocompatibility of transfer
method
+
+++
+++
++
++
Actin functionality
+++
+++
+++
+++
+++
Safety Consideration
Biosafety Level
BSL1
-
-
BSL2
BSL2


1 Not all of these combinations have been tested in all indicated cell types. We encourage you to use this table as a guideline to understand how the LifeAct system can be optimally applied.


* Original LifeAct publication in Nature Methods:
Riedl J, et al. Lifeact – a versatile marker for the visualization of F-actin. Nature Methods 5, 605-607 (2008)

LifeAct is a registered trademark of ibidi GmbH.

For license and product use information please refer to www.ibidi.com, Legal Notes.

Supporting Material

Movies

7 Related Products