DIC Lid for µ-Dishes

Applications

• Differential Interference Contrast (DIC) microscopy 
• Sterile cell culture
• Live cell imaging

Specifications

Technical Features

• Lid with glass coverslip insert
• Low birefringence for excellent DIC imaging
• Compatible with all ibidi 35 mm µ-Dishes
• Not compatible with the Glass Bottom Dish ^{35 mm}

The DIC Beam Path

For excellent imaging with a differential interference contrast (DIC) microscope, all elements in the optical pathway need to be DIC-compatible. They must exhibit a low birefringence, meaning that there is only low interference with the polarized light. Just one single incompatible element in the beam path of the transmitted light would destroy the orientation of the polarized light, leading to disturbance of the DIC imaging effect. When using the ibidi µ-Dishes combined with the special DIC Lids for µ-Dishes , excellent DIC images can be generated.

DIC Compatibility of Different Materials

Experimental Example

Human keratinocytes on an ibiTreat surface. Imaging with a DIC objective lens 10x.