Wound Healing | Migration | Culture-Inserts

What are the experimental differences between impedance-based wound healing assays and the use of Culture-Inserts?
Why do the cells sometimes detach from the coverslip when an ibidi Culture-Insert is removed?
How can you minimize cell division in migration experiments?
Will the coating protein become inactive when drying occurs on the Culture-Insert?
Have you tried a PORN/laminin coating with Culture-Inserts?
Is there a way to do wound healing assays with cell suspensions, or does the assay only work for adherent cells?
Is a coating protocol available for experiments using Culture-Inserts?
Have you ever had trouble getting Culture-Inserts to stick to custom-coated surfaces?
What keeps the Culture-Inserts from leaking during cell seeding?
Can I use the Culture-Inserts on cover glasses?
How should I store the Culture-Inserts?
Are the Culture-Inserts reusable?
Sometimes it is difficult to see the confluency of a cell layer in the Culture-Insert. Do you have any suggestions?
Sometimes, the cells tend to clump at the edges of the ibidi Culture-Insert. How can I avoid this?
How can the ibidi Culture-Insert mimic a physiological wound without causing cell damage?
Are the cells damaged when creating a gap with the Culture-Insert?
Is it possible to make wounds smaller than 500 µm with a Culture-Insert?
How is the Culture-Insert fixed onto the dish?
Can the Culture-Inserts be used on protein-coated surfaces? Will the coating be disturbed by pulling out the Culture-Insert?
How many cells do you have to seed in a Culture-Insert for a cell migration experiment?

Is there a way to do wound healing assays with cell suspensions, or does the assay only work for adherent cells?

At the moment, the wound healing assay using the Culture-Insert can only be used for adherent cells. However, migration studies of single cells can be performed in the µ-Slide Chemotaxis by embedding the cells into a three-dimensional matrix (e.g., a Collagen Type I gel).

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Wound Healing | Migration | Culture-Inserts

µ-Slide 8 Well high

µ-Slide I Luer

µ-Plate 96 Well Square

µ-Slide 8 Well high Glass Bottom

µ-Dish 35 mm, high

Is there a way to do wound healing assays with cell suspensions, or does the assay only work for adherent cells?

Is there a way to do wound healing assays with cell suspensions, or does the assay only work for adherent cells?

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Wound Healing | Migration | Culture-Inserts

µ-Slide 8 Well high

µ-Slide I Luer

µ-Plate 96 Well Square

µ-Slide 8 Well high Glass Bottom

µ-Dish 35 mm, high

Is there a way to do wound healing assays with cell suspensions, or does the assay only work for adherent cells?

Necessary

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