Have you tried a PORN/laminin coating with Culture-Inserts?
So far, we have not tested this coating with Culture-Inserts, but you can request free samples from ibidi to test this coating in your own lab.
Wound Healing | Migration | Culture-Inserts
- What are the experimental differences between impedance-based wound healing assays and the use of Culture-Inserts?
- Why do the cells sometimes detach from the coverslip when an ibidi Culture-Insert is removed?
- How can you minimize cell division in migration experiments?
- Will the coating protein become inactive when drying occurs on the Culture-Insert?
- Have you tried a PORN/laminin coating with Culture-Inserts?
- Is there a way to do wound healing assays with cell suspensions, or does the assay only work for adherent cells?
- Is a coating protocol available for experiments using Culture-Inserts?
- Have you ever had trouble getting Culture-Inserts to stick to custom-coated surfaces?
- What keeps the Culture-Inserts from leaking during cell seeding?
- Can I use the Culture-Inserts on cover glasses?
- How should I store the Culture-Inserts?
- Are the Culture-Inserts reusable?
- Sometimes it is difficult to see the confluency of a cell layer in the Culture-Insert. Do you have any suggestions?
- Sometimes, the cells tend to clump at the edges of the ibidi Culture-Insert. How can I avoid this?
- How can the ibidi Culture-Insert mimic a physiological wound without causing cell damage?
- Are the cells damaged when creating a gap with the Culture-Insert?
- Is it possible to make wounds smaller than 500 µm with a Culture-Insert?
- How is the Culture-Insert fixed onto the dish?
- Can the Culture-Inserts be used on protein-coated surfaces? Will the coating be disturbed by pulling out the Culture-Insert?
- How many cells do you have to seed in a Culture-Insert for a cell migration experiment?