Transfection, Transduction, and Membrane Fusion

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Transfection of eukaryotic cells is the process of inserting plasmid DNA or RNA (e.g., siRNA) into these cells. As pure genetic material would not naturally be transported into cells, an efficient transfection method is necessary.

Non-chemical methods, such as electroporation, or particle-based techniques may be used. However, these methods require high investments in expensive technical equipment. In contrast to these techniques, transfection of eukaryotic cells with a transfection reagent like TorpedoDNA or Torpedo siRNA is a very easy, cost-effective, and efficient method for introducing foreign genetic material into cells.

In addition to transient transfection, researchers also rely on the stable transfection of cell lines, which leads to long-term gene expression and the stable synthesis of the protein of interest that was introduced.

Transfection of eukaryotic cells with plasmid DNA (here pLifeAct)


  • Imaging of fluorescent proteins after plasmid transfection (e.g., visualization of F-actin after transfection with pLifeAct)
    For more information please watch our movie: LifeAct: Actin Marker for Live Cell Imaging
  • Protein localization with fluorescent labels
  • RNA silencing by siRNA
  • Gene regulation studies using miRNA

Microscopic analysis of transfection experiments is an easy method for analyzing transfection efficiency, or for directly monitoring the effects and localization of synthesized proteins. ibidi’s µ-Slides VI 0.4 allow for cell culture, transfection, and microscopy to occur all in one slide. This makes them perfect for applications like imaging of fluorescent proteins, protein localization, or gene regulation studies.

Apart from utilizing the perfect culture slide, the success of transfection experiments can be enhanced by using the optimized reagent in optimized amounts. When building the DNA lipid complexes the ratio of DNA/RNA and transfection reagent is crucial.

Torpedo Transfection Reagents

With the Torpedo Transfection Reagents, ibidi provides the ideal reagents – cationic lipids – and protocols to successfully perform transfection experiments directly in the ibidi µ-Slides and µ-Dishes. Torpedo DNA was optimized specifically for the transfection of mammalian cells with plasmid DNA in ibidi’s µ-Slides. Torpedo siRNA enables successful gene silencing experiments when using ibidi’s µ-Slides in live cell imaging. Both reagents combine low toxicity with outstanding transfection results and a simple, rapid protocol.

ibidi Movie

View a detailed transfection protocol for adherent cells in an ibidi microscopy chamber: Cell Transfection Using Torpedo DNA (MV 24)