Stage Top Incubator

Cell Biology

The immortalized human bronchial epithelial cells HBEC3-KT (ATCC) were grown on the ibidi μ-Slide 8 Well (ibiTreat) chambered coverslips. The cells were transduced with an shRNA lentivirus to knock down the gene of interest, resulting in multinucleation. Images were obtained using a 60x objective on an inverted Nikon A1R confocal microscope.

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