Cells react sensitively to changes in their environment. Factors such as temperature, humidity, and CO2/O2 levels significantly influence the outcome of cell culture assays. In order to achieve biologically relevant and reproducible results, it is crucial to maintain optimal conditions on the microscope stage during live cell imaging experiments.
To enable high-quality live cell imaging under physiological conditions, the following parameters need to be considered:
Environment:
- Temperature
- Carbon Dioxide (CO2) Levels and pH of the Medium
- Humidity and Evaporation
- Oxygen (O2) Levels
Optical Aspects:
ibidi Solution
The ibidi Stage Top Incubation Systems are available for slides/dishes and for multiwell plates. They allow for accurate and reliable control of important live cell imaging parameters (temperature, CO2 and O2 concentration, and relative humidity) during short-term and long-term assays on any inverted microscope.
