Stage Top Incubator

Cell Biology

Polarized primary rat hepatocytes were immunostained against the tight junction protein ZO-1 (yellow). Actin filaments (red) and nuclei (green) were stained with fluorescently labeled phalloidin and DAPI, respectively. The image is a projection of a z-stack acquired using a Leica TCS SP5 confocal microscope system with a 100x objective.