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Judith Bain, Neil Gow, Lars Erwig
University of Aberdeen, Aberdeen, United Kingdom
RAW264.7 murine macrophage cell line stained with TRITC-phalloidin, phagocytosing Candida albicans filamentous fungus stained for yeast cell wall with FITC and cell wall chitin with Calcofluor white. Macrophages and fungi were incubated in supplemented DMEM medium for 2 hours in an ibidi μ-Slide 8 Well prior to fixing and staining. Cells were imaged in 3D using a Nikon UltraVIEW VoX spinning disk microscope and Volocity® software.
Published in the 2015 ibidi Calendar.
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