Stage Top Incubator

3D Cell Culture

Spheroid of human intestinal fibroblasts and outgrowth endothelial cells sprouting in a fibrin hydrogel, cultured in an ibidi µ-Slide 4 Well. Endothelial cells were stained with CD31 (yellow), fibroblasts with vimentin (red), and DNA with DAPI (cyan). This image was acquired using a Leica TCS SP5 II confocal laser scanning microscope with a 10x objective.