Stage Top Incubator
3D Cell Culture
Department of Microbiology, University of Osnabrück, Germany
3D murine ileum organoid cultured in an ibidi µ-Slide 8 Well coated with Matrigel®. Phalloidin (green) was used to stain F-actin to visualize the orientation of polarized epithelial cells. Nuclei were labeled with DAPI (blue) and the plasma membrane was stained with CellMask (red). The cells were imaged with a Zeiss Cell Observer spinning disc confocal microscope using a 40x objective.
Published in the 2022 ibidi Calendar.
