Over more than a decade, ibidi has earned the trust of customers worldwide.
Michele Chiappi, Imperial College, London, United Kingdom
"I have tested the slides with 8 wells with detachable soft silicon wells (8 Well Chamber, removable). They are amazingly easy to use and then easy to mount a coverslip and do confocal microscopy. I love them."
Johanna Hol Fosse, Norwegian Veterinary Institute, Oslo, Norway
I have tested the µ-Slides 8 Well on adherent fish cell lines, originally isolated from salmon head kidney (ASK and SHK cells) and have been very happy with them. The ASK cells preferred the ibiTreat slides, while the SHK cells grew well on both ibiTreat and Collagen IV. I also tested the ASK cells in a µ-Slide 18 Well - Flat, and that worked well, provided that the medium (L-15, 10% FCS) was changed at least every other day. Cells were seeded 5,000/well in the µ-Slide 18 Well - Flat and 50,000/well in the µ-Slide 8 Well. I will place an order within the next few weeks.”
Johanna Hol Fosse
Norwegian Veterinary Institute
Jana Kudova, Czech Academy of Sciences, Brno, Czech Republic
"I had a chance to test the Culture-Insert 2 Well in a µ-Dish with FaDu cells (a hypopharyngeal carcinoma cell line), and I have to say that it works perfect. Before, I did a scratch assay using pipette tips. It was very difficult and the reproducibility was low. When using the ibidi Culture-Insert, all issues disappeared. I have recommended your product to other colleagues and we plan to use it as a routine method in our lab."
Czech Academy of Sciences
Tan Hop Huynh, University of Basel, Biozentrum, Basel, Switzerland
"I checked the interaction of bacteria and host cells by monitoring the fluorescence signals in the time-lapse experiment. Your µ-Slide 18 Well Flat did a great job to bring property and clear results."
Tan Hop Huynh
University of Basel
Yuki Manabe, Kyoto University, Graduate School of Agriculture, Kyoto, Japan
"Thank you for your great product µ-Slide 8 Well. I think this is the best to obtain fluorescent images of adherent cells such as RAW264 and RBL-2H3 using oil immersion microscopy. Thanks to this slide, we can get various good results."
Graduate School of Agriculture
Michelle Somerville, Cardiff University, Cardiff, United Kingdom
"We have tested the µ-Slide Angiogenesis and the µ-Slide 8 Well and have found them to be great products. The slides are being used for imaging fluorescently labelled organoids using confocal microscopy and we will continue to use them. I have already recommended them to a lab colleague and will continue to recommend them to others."
Milena Leseva, The Stephan Angeloff Institute of Microbiology, Sofia, Bulgaria
"The 12 Well and 8 Well Chambers, removable worked very well. We used them for fluorescence staining for H4K16ac in non-adherent, neutrophil cells. We treated the cells with different compounds that are expected to change this modification.
Overall, we are extremely happy with how easy it is to use the cell culture chambers, especially when you have cells with different treatments. We liked the fact that the chamber format minimizes the volume of antibody that we required in order to get a good signal."
The Stephan Angeloff Institute of Microbiology
Francesca Sartor, Ludwig-Maximilians-University, München, Germany
"I tested the dishes with glass bottom for fluorescence microscopy applications. We used U20S cells, plated on the ibidi Glass Bottom Dish 35 mm, and performed time-lapse imaging. I found the quality of the samples very good. We could get sharp microscopy pictures."
Paola Antonello, Institute for Research in Biomedicine, Bellinzona, Switzerland
"I tested the μ-Slide Angiogenesis to study the chemokinesis of lymphoma cells in a 3D migration using collagen as a matrix in the presence of the chemoattractant. I used these slides to test different concentrations of bovine collagen, in order to optimize my protocol. The slides worked perfectly. I tested the rate of chemokinesis in an ImageXpress confocal microscopy for 6 hours and obtained a very good resolution with 4X magnification using phase contrast."
Institute for Research in Biomedicine
Patrick Nijman, Technical University Delft, The Netherlands
"I am using various types of the µ-Slide I Luer under flow conditions to study Schistosomiasis haematobium eggs in urine flow for diagnostic purposes. I am very satisfied with the product! These slides have been of great help so far. Actually, my supervisor and I are thinking of maybe ordering a large amount of Luer slides (>100) in the future."
Technical University Delft
Christian Ploner, Medical University of Innsbruck, Austria
"We tested the µ-Plate 96 Well for long-term, in-vitro differentiation experiments with human adipose derived stem cells (hASC). In our hands, this plate type is the only one (compared to other optical plates) where the cells differentiate properly, and which was qualified for high throughput screening with a confocal scanning microscope."
Medical University of Innsbruck
Serene Gwee, National University of Singapore, Singapore
"I started using the Glass Bottom chambered slides with my time-lapse imaging of zebrafish embryos and they have been very useful and applicable for my experiments."
Sze Ling Gwee
National University of Singapore
Department of Biological Sciences
Lee Shu Ying, National University of Singapore, Singapore
"As a microscopy core facility, we have to recommend the most suitable imaging plates to our users for their experiments. The µ-Slide 8 Well is our best seller amongst the other wonderful platforms that ibidi carries.
We had multiple experiments where the cells/bacteria were not adhering well to glass coverslips, but grew perfectly on the ibiTreat surface. Confocal images turned out great as well. Our users also love how the cover fits snugly for ease of handling."
Lee Shu Ying
National University of Singapore
Yong Loo Lin School of Medicine
Susanne Proksch, Medical Center–University of Freiburg, Freiburg, Germany
"The µ-Slide Chemotaxis made it possible to study stem cell-mediated chemoattraction of tissue cells, which was realized in a co-culture experiment. I was thrilled with the deliberate system and the convenient all-in-one package supplied by ibidi, including training classes and online courses on how to set up the experiment and how to analyze the data, all with perfect individual customer support. From my point of view, the method is pretty fancy and the µ-Slide Chemotaxis system is very sophisticated, allowing for in vivo-like insights into cell behavior for high-impact publications."
Rachid Rezgui, New York University Abu Dhabi, Abu Dhabi, UAE
"We have successfully tested the µ-Slide 4 Well and 8 Well Glass Bottom. We are very satisfied with these slides. First of all, the fast delivery allows us to be more flexible when we plan our experiments. We tried Fluorescence correlation spectroscopy to study the interaction of Cell Penetrating Peptides with small interfering DNA and obtained great results. As FCS measures the concentration in the focal volume, it is extremely sensitive to surface changes. As we used glass bottom dishes, we were able to achieve a very small focal volume for maximum precision. We also used them for confocal imaging on Mouse Embryonic Fibroblasts (GSK3-KO MEF) to study molecular motors and obtained great images on a Leica SP8 with Hyvolution."
Ali Alhoshani, King Saud University, Riyadh, Saudi Arabia
“The ibidi Culture-Insert 2 Well 24 plate was unbelievably helpful when I wanted precise and consistent results for wound healing assay. It saved my lab so much time and effort. The web-based quantitative image analysis feature is a huge plus. Thank you for such useful products. "
King Saud University
Department of Pharmacology and Toxicology
Contu Raluca, National Center of Neurology and Psychiatry, Tokyo, Japan
"We often use ibidi glass bottom dishes for live imaging of cultured cells. We were able to get very good images of protein delocalization, as well as of nucleic acids taken up by living cells. The optical quality of the bottom of the dishes is extraordinary. Actually, two of our papers for which we used ibidi µ-Dishes and μ-Slides were published in 2017:
Contu et al. 2017 J Cell Sci. http://jcs.biologists.org/content/130/17/2843.long
Takahashi et al. 2017 RNA Biology http://www.tandfonline.com/doi/full/10.1080/15476286.2017.1302641"
National Center of Neurology and Psychiatry
Pierre-Olivier Bagnaninchi, MRC Centre for Regenerative Medicine, University of Edinburgh, UK
"ECIS made it happen: without impedance sensing we will never have been able to look at barrier function in the liver in addition to pure toxicity. This unique feature of the ECIS Zθ allowed us to shed a new light on how the common painkiller paracetamol (acetaminophen) causes liver damage." (http://www.nature.com/articles/srep37541)
MRC Centre for Regenerative Medicine
University of Edinburgh
Christine Neumann, Institute of Medical Biology, A*STAR, Singapore
"The Fuse-It-mRNA kit was easy to use and worked very well for the N/TERT-1 immortalized skin cells I transfected. At the time of transfection, keratinocytes were 70% confluent and showed excellent viability after transfection. The GFP became visible two hours after transfection and lasted for more than one week while keratinocytes were undergoing differentiation. With an efficiency of more than 50% positive cells and unaffected cell morphology, it is an unbeatable alternative to electroporation or lipofection techniques used for transient transfection with our difficult to transfect keratinocytes."
Institute of Medical Biology
Astrid König, Senckenberg Museum of Natural History, Görlitz, Germany
"We use the μ-Slide 8 Well Grid-500 for tracking experiments of tardigrades and nematodes. We are really thrilled about the product. The scaling of the slide helps us very much in evaluating the movement of the animals during the experiment."
Please find more details at:
Senckenberg Museum of Natural History
Max-Joseph Kraus, Praxis Geiselgasteig, Grünwald, Germany
"I really love these slides! They are the best that happened to my research workflow. I mainly use the removable 12-well chambers for the high resolution dark field microscopy of platelets and they work excellent for me."
Henrike Rebl, University Medicine, Rostock, Germany
"I have tested the Culture-Insert 2 Well and 3 Well and they worked quite nicely. I will definitely use the ibidi Culture-Inserts and µ-Dishes again because they are so easy to use and they work great for fluorescence microscopy."
Petra Weber, Aalen University, Optical Engineering, Aalen, Germany
"We have tested the Removable Chambers (3 Well and 8 Well) and were very happy. Due to our very sensitive TIRF microscopy with an upright microscope we need appropriate glass slides with removable chambers. We tested adherent HeLa cells to detect Förster Resonance Energy Transfer between EGFR-CFP and Grb2-YFP with Fluorescence Lifetime Imaging. In particular for the activation or inhibition of intracellular signaling pathways, we need the subdivisions on the object slide. It was very easy to remove the chambers. Thank you very much for the possibility to test the chambers for free. I am sure I will use them again."
Daniel Kummer, ZMBE, Institute for Medical Biochemistry, Münster, Germany
"I tested the 8 Well Chamber, removable, and was very happy with the results. The system worked flawlessly. The cells grew very well and the immunofluorescence stainings were of good quality. In general, our institute has always had good experiences with ibidi products. It was the same with the 8 Well Chamber, removable. I have already recommended it to my colleagues."
ZMBE, Institute for Medical Biochemistry
Sofia Oke, University of Toronto, Department of Immunology, Toronto, Canada
“I have had the chance to try the µ-Slide 8 Well Grid-500 and have been very impressed. I have been using the slides to perform assays with 3D organoid cultures. The µ-Slide 8 Well Grid-500 was very useful and easy for me to monitor my organoids as they grew. I was performing an assay where I irradiated my organoids and monitored recovery. I am very impressed with these products and will be sure to keep in touch if I once again need them in the months to come.
University of Toronto, Department of Immunology
Gisa Gerold, TWINCORE – Center for Experimental and Clinical Infection Research, Hannover, Germany
“We tested the Culture-Inserts 2 Well and now use them successfully on human hepatoma cells (Lunet N3 hCD81 cells, which are derived from Huh-7.5 cells). We are very satisfied with the ibidi products and with the fast delivery of the test chambers.”
TWINCORE – Center for Experimental and Clinical Infection Research
Louise Ince, Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilian-University, Munich, Germany
"I used the Fuse-It-mRNA kit to label mature murine bone marrow-derived dendritic cells with GFP and was very happy with the results. The kit was quick and easy to use, and is an excellent non-viral transfection method for these cells with only a sonicator required.”
Walter Brendel Centre of Experimental Medicine
Stefan Zahler, Pharmaceutical Biology, Ludwig-Maximilians-University, Munich, Germany
“Good cell viability and good transfection efficiency, at the same time, are hard to get with HUVECs. Shuttling mRNA directly into the cells with Fuse-It-mRNA gave a very good efficiency (> 60% positive cells), and left the cells in an excellent state. This approach might be the long-desired alternative to using plasmids in cells which are hard to transfect.”
Moritz Schneider, Ludwig-Maximilians-University, München, Germany
"We could successfully apply Fuse-It-mRNA for transfection of A549 and MDCK cells. The protocol was very easy to follow, and the transfection efficiency exceeded our expectations!”
Madeleine Zerbato, Brigham Regenerative Medicine Center, Cambridge, USA
"The ibidi µ-Slide 4 Well and µ-Slide 8 Well that I am working with are actually the best products I have tested so far for cultivating D3 mouse embryonic cell lines. They attach well to the treated slide (ibiTreat µ-Slides), and the resulting morphology is very similar to the one they have on regular plastic. That is something very unique to those microscopic slides, since D3 mESCs do not attach to glass and form embryoid-like structures. I tested other non-glass slides from other manufacturers but the ibiTreat µ-Slides are the only ones that give me a physiological-like morphology.”
Brigham Regenerative Medicine Center
Des Field, University College Cork, Ireland
"I'm glad to say that the ibidi plates were ideal for our purposes. I am working with bacteria (Staphylococci and Pseudomonas) that form biofilms on surfaces. They easily form biofilms in regular 96 well polystyrene microtiter plates but these cannot be visualized by confocal microscopy. We are using combinations of antibiotics to eradicate these biofilms and wished to use live/dead fluorescence staining and confocal microscopy to visualize the results. We set up the experiment using the ibidi µ-Plate 96 Well and we were able to observe perfectly the results of our experiments. We will be hoping to upscale this type of work in the near future (given the quality of the results we have achieved) and so will hope to be ordering more of these microtiter plates in the near future.”
University College Cork
Barbara Fogli, Medical University Innsbruck, Austria
"I tested the µ-Slide 4 Well for the culturing of mice DRG neurons. I found them particularly useful for my experiments, since they allow me to have different conditions within the same slide, and to directly do staining and microscopy within the same slide.
After testing them, I decided to order a box of the µ-Slide 4 Well Ph+, because the plastic layer in the middle of the wells allows me to have a more homogeneous distribution of the neurons, which is optimal for microscopy.”
Medical University Innsbruck
Jessica Davis, University College Dublin, Ireland
"I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment—I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”
University College Dublin
Catarina Leite Pereira, University of Porto (INEB), Porto, Portugal
"I have done some experiments using the µ-Slide 2 Well and µ-Slide 4 Well for static culture ex vivo, and imaging of intervertebral discs at the IN Cell Analyzer. They work perfectly! Thank you very much, these slides are really very useful, since I have been struggling for some time trying to find the appropriate support to visualize this kind of sample.”
Catarina Leite Pereira
University of Porto (INEB)
Renate Gehwolf, Paracelsus Medical University, Salzburg, Austria
"We are currently using the Culture-Insert to perform wounding experiments with somatic stem cells, and also the 2D chemotaxis slides to study the migratory behavior of these cells. We are very happy with the products, because they are user-friendly, especially for wounding assays, as we do not want to do scratching because of the inevitable high variability. However, with the ibidi Culture-Inserts, we can perform highly reproducible experiments. We will definitely use them again.”
Institute of Tendon & Bone Regeneration
Paracelsus Medical University
John Fassett, University of Graz, Austria
"The µ-Slides 8 Well are great! I really like the ones with the grid. We were able to use them for the live cell imaging of transfected GFP and RFP fusion proteins and could see some really good images.”
University of Graz
Maros Mastrak, Medical University Innsbruck, Austria
"I tried your mounting medium, which worked really great. I had an older one from a different manufacturer, but it caused very high background levels. However, with the ibidi Mounting Medium, I was able to save at least half of the exposure times and gain much clearer results."
Medical University Innsbruck
Kenneth Martin, University College Cork, Ireland
"Thank you for the great products that you have developed over the years. The ibidi Pump System has immeasurably helped our research. We have recently published a paper in “Stem Cells” that uses your flow system, the µ-Slide I Luer, and the µ-Plate 96 Well very effectively. It seems to me that ibidi are constantly coming up with clever and useful products, and I like to keep an eye on what interesting things are coming out next from them."
University College Cork
Stefanie Kirchberger, Children's Cancer Research Institute, Vienna, Austria
"We tested the µ-Slide 2 Well and µ-Slide 8 Well in the uncoated version for the confocal microscopy of fish larvae. Both types of slides worked fine on our confocal microscope. We have already ordered another bunch of those slides for the lab!"
Children's Cancer Research Institute
Tim Sanchez, Harvard University, Cambridge, USA
"For my research, I need to take time-lapse images of mouse embryos developing from the 1 cell stage all the way to 5-day blastocyst. Anyone who has worked with mouse embryos knows they are incredibly sensitive and delicate, so we required an extremely stable environment over several days. I struggled for months with a different system, but the ibidi Heating Stage worked very quickly. I was immediately impressed with the quality. Calibration with the included thermocouple was easy and accurate. Now I get consistent development every time, and the percentage of embryos developing to blastocysts is the same as the control incubator! Terrific product, thanks ibidi.”
Emily Lynes, German Centre for Neurodegenerative Diseases, Tübingen, Germany
"Thanks for providing such a great practical course on chemotaxis assays. I have subsequently been successful in optimizing a chemotaxis protocol for THP-1 immune cells! I am sure I never would have managed to set up this experiment at all without your help."
German Centre for Neurodegenerative Diseases
Peter Krenn, Laboratory for Immunological and Molecular Cancer Research, Salzburg, Austria
“Investigating the homing of primary human chronic lymphocytic leukemia cells in NOD-SCID mice has always been a demanding experimental approach due to the delicate cells and the high working load. Using Fuse-It-Color dyes, we were able to label the cells in an easy, quick, and robust manner, which, at the same time, did not interfere with the homing capacities of the cells, thereby making a complex experiment easier.”
Laboratory for Immunological and Molecular Cancer Research
Stephane R. Gross, Aston University, Birmingham, United Kingdom
“For my group, the efficient labeling of cells is an extremely important issue. Since most of our cells are very sensitive to changing medium conditions, mild and fast labeling is a must. We have found, in the very short time we have used the system and therefore without real optimization, that both the Fuse-It-Color and Fuse-It-P were very well functional, with the latter perfectly capable of delivering our target proteins into HeLa cells. Such delivery was obtained using relatively low amounts of exogenous proteins and incubation times as short as 5 minutes.”
Dr. Stephane R. Gross
Lecturer in Cellular Biology
School of Life and Health Sciences
Aston University Birmingham
Jing Zhiao Garland, University of Cambridge, United Kingdom
"Thank you very much for organizing the Training Course. It was very helpful. We all enjoyed the course very much and I felt I was energized after attending this training. A big special thank you for letting me rediscover the great value of the ibidi website."
Jing Zhao Garland
University of Cambridge
Department of Medicine
Michael Börsch, Jena University Hospital, Friedrich Schiller University, Jena, Germany
"The incorporation of transmembrane proteins from proteoliposomes or detergent-containing buffers into black lipid membranes is a very dicey issue. I have used your high fusogenic Fuse-It-P for this purpose and it really worked perfectly. It was an extremely simple preparation, and the incorporation was very reliable and easily adjustable. It made this very difficult step for all experiments in my institute much easier. Thanks a lot."
Prof. Dr. Michael Börsch
Single-Molecule Microscopy Group
Jena University Hospital
Friedrich Schiller University Jena
Ivan Bedzhov, University of Cambridge, Gurdon Institute, Cambridge, United Kingdom
“We looked for an imaging-compatible material that supports embryo attachment. This led us to establish that we can successfully grow and image mouse embryos on ibiTreat μ-Plates 24 Well and µ-Slides 8 Well from ibidi, which have optical properties similar to glass, and allow efficient blastocyst attachment. Thus, ibidi slides and plates are now an indispensable part of our in vitro culture methodology.“
University of Cambridge
Reinhard Windoffer, Institute for Molecular and Cellular Anatomy (MOCA), University Hospital RWTH Aachen, Germany
"The incorporation of magnetic beads into cells for use in our magnetic tweezers experiments was extremely difficult and stressful for our cells. With Fuse-It-Beads, we can now easily perform the incorporation within a few minutes, making our experiments much easier and more reliable."
Dr. Reinhard Windoffer
Institute for Molecular and Cellular Anatomy (MOCA)
University Hospital RWTH Aachen
Anuradha Vajjala, Nanyang Technological University, Singapore Centre on Environmental Life Sciences Engineering, Singapore
"To study the ultra structure of Streptococcus pyogenes biofilm grown on mouse embryonic fibroblasts, I used the ibidi Treat 35mm μ-Dish to optimize the right conditions of biofilm growth. Simply awesome! It has made life so much simpler for me. Tissue culture combined with microscopy is so much easier now. The dishes are very user-friendly too. I have used the ibidi products to get very good quality images. I am really happy with the products!"
Nanyang Technological University
Singapore Centre on Environmental Life Sciences Engineering
Dr. Christiane Wiesner, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
“We use LifeAct-TagRFP and LifeAct-TagGFP2 plasmids in primary human macrophages. The LifeAct probes put us in the position to detect F-actin-rich adhesion structures, without the drawback of disturbing their dynamics. The signal is bright and clear without the background of nonintegrated G-actin. LifeAct gives us the opportunity to highlight F-actin-enriched, cytoskeletal organizations, without the disadvantages of fluorophore-tagged actin overexpression.”
Dr. Christiane Wiesner
University Medical Center Hamburg-Eppendorf
Randy Van Der Ploeg, St. Boniface Research Centre, Winnipeg, Manitoba, Canada
“We are pleased with the ibiTreat 35 mm culture dishes. I have given some of your product to one of our grad students to try and he is amazed with the robust axonal outgrowth that he finds only after one day of seeding cells. I have tested other dishes from your competitors and I did not get the reproducibility in growth characteristics like the ibiTreat µ-Dish.
The 35 mm dish holds up wonderfully to automated scanning over a wide scan area (essentially 98% of the growth surface area) which demonstrates the optical clarity and focal stability of the surface over such a wide area (1296 scan fields per well). I look forward to using more of the ibidi product lines in the future.”
Randy Van Der Ploeg
Division of Neurodegenerative Disorders
St. Boniface Research Centre
Kaela Varberg, Indiana University, School of Medicine, Indianapolis, USA
“The µ-Slides Angiogenesis created by ibidi have significantly increased the efficiency and accuracy of the angiogenesis assays in our lab. Using the ibidi slides not only reduces the amount of time spent capturing images, but it also reduces the time it takes to quantify the data since the structures are in the same focal plane. Another added bonus is the reduced amount of matrigel required for each experiment which saves money!”
School of Medicine
Shane R. McSweeney, Cardiovascular Division, BHF Centre of Research Excellence, School of Medicine, King’s College London, United Kingdom
"The µ-Slide I Luer facilitates the culture of endothelial cells under defined complex flow patterns. We are particularly interested in the effects of oscillatory shear stress on redox signaling pathways, and the ibidi Air Pump and Fluidic Units are ideal systems for this purpose. The µ-Slides are convenient for both live and fixed cell imaging as well as the collection of protein and RNA samples and other cell analyses. The system has allowed us to easily investigate physiological shear patterns in vitro without a background in engineering.”
Shane R. McSweeney
BHF Centre of Research Excellence
School of Medicine
King’s College London
Judith Cathcart, Advanced Optical Microscopy Facility, University Health Network, Toronto, Canada
"The ibidi chambers (Culture-Inserts) we used in our wound healing assays helped to keep the cell free area clean and consistent in size without damaging the ECM or leading edge of the cell fronts, making analysis of results more manageable and comparable. They're convenient and easy to use and their small size helps to conserve precious cells and culture materials. We'd certainly use them again.“
Advanced Optical Microscopy Facility
University Health Network
Jason W. Bjork, PhD, 3M Corporate Research Materials Laboratory, St. Paul, USA
“The ibidi Culture-Inserts for wound healing assays provide a new line of testing for us. We wouldn't do traditional scratch assays at all because of the inherent variation, but with the new inserts we're able to provide many images to support our research. Our results are repeatable and reliable regardless of which researcher conducts the assay.”
Jason W. Bjork
3M Research Materials Laboratory
Ross Flockhart, PhD, Stanford School of Medicine, Stanford, USA
"The ibidi cell migration Culture-Inserts enabled me to perform highly reproducible and quantitative migration experiments. Since physical scratching isn't required, uniformity is maintained and adverse wounding responses are not an issue.
The online quantification tool WimScratch for the assay is also fantastic and it yields quantitative data from the images extremely quickly with little effort required. I'd definitely recommend it for migration studies.“
Ross Flockhart, PhD
Stanford School of Medicine
Department of Dermatology
PD Dr. Peter J. Hanley, University of Münster, Münster, Germany
"In the past 5 years, we have extensively used the ibidi µ-Slide Chemotaxis 2D to perform time-lapse images of primary mouse macrophages migrating in a chemoattractant gradient. We are most grateful that this device was developed, otherwise it would have been technically difficult to perform robust chemotaxis assays over long time periods (in our case, up to 14 h). By adding a little food coloring dye to the chemoattractant solution one can observe that the dye requires days to equilibrate with the opposing reservoir. Gradient kinetics can be measured by substituting the chemoattractant for a fluorescent dye (with similar molecular weight) and taking serial confocal fluorescence images in the observation area, the narrow channel which connects the two reservoirs.“
PD Dr. Peter Hanley
University of Münster
Institute of Molecular Cell Biology
Dr. Juliane Merl, Helmholtz Zentrum München, Germany
„We used the Torpedo siRNA Transfection Reagent for the transfection of human retinal pigment epithelial cells and experienced a reproducible and efficient knock-down of our protein of interest; even with really low amounts of siRNA.“
Dr. Juliane Merl
Research Unit Protein Science
Helmholtz Zentrum München
Loïc Dupré, INSERM, Toulouse, France
"The ibidi µ-Slides Chemotaxis 2D and 3D have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.
I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"
Loïc Dupré PhD
INSERM UMR 1043
Purpan University Hospital
Sam Noppen, Rega Institute for Medical Research, Leuven, Belgium
"I used the ibidi µ-Plate Angiogenesis 96 well for bioluminescence measurements. The plate performed exceptionally well for this specific application, as it reduced my sample volume drastically and the optical quality was just superb."
Rega Institute for Medical Research, KU Leuven
Joanne Marrison, University of York, UK
"I plated and transfected cells on the ibidi µ-Slide 8 Well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are 'slide' sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great."
Imaging and Cytometry Laboratory (B/K051)
Bioscience Technology Facility, Department of Biology
University of York, York
Matthew Taylor, PhD, Princeton University, Princeton, USA
"The 35 mm µ-Dish is invaluable for high resolution imaging performed in support of my work. The optical plastic is fantastic substrate for building compartmentalized neuronal cultures. Thank you for making great products.”
Department of Molecular Biology
Dr. Esther G.L. Koh, National University of Singapore, Singapore
“ibidi made it much simpler for me to prepare cells for confocal microscopy and live-cell timelapse microscopy. Cells that attached poorly to glass grew better on ibidi µ-Slides/Dishes. The µ-Slide Angiogenesis allowed me to minimise the amount of cells and reagents required for an experiment.”
Dr. Esther G.L. Koh
Head, Advanced Imaging Laboratory
Life Sciences Institute Immunology Programme
National University of Singapore
Centre for Life Sciences
Dr. Nynke van den Akker, Maastricht University, Maastricht, The Netherlands
“We've been working with the ibidi Pump System and Labware for over 5 years now and have recommended it to numerous colleagues as well. The plastics are ideal for cell culture and all forms of imaging, including automated imaging (easy to focus) and all sorts of fluorescence (clear, optically superb plastics). The ibidi Pump System in fact made the endothelial cell under flow the default of our lab! And above all, the technical support is always fast and efficient.”
Dr. Nynke van den Akker
Department of Cardiology
Owen McCarty, PhD, Oregon Health & Science University, Portland, USA
“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”
Owen McCarty, PhD
Department of Biomedical Engineering
Oregon Health & Science University
James J. Faust and David G. Capco, Arizona State University, Tempe, USA
“ibidi has made the tedious process of correlated light- and scanning electron microscopy remarkably simplified with their µ-Dishes 35mm, high glass bottom Grid 500. This product combines all of the essential elements for high quality imaging: optically flawless glass essential for high resolution light microscopy, media reservoirs suited for a variety of imaging systems, and a grid system to rapidly relocate specimens in the scanning electron microscope. This product has made the difficult easy.”
James J. Faust and David G. Capco
Molecular and Cellular Biosciences
Arizona State University
Dr. Thomas A.J. McKinnon, Imperial College London, UK
„I work with the ibidi slides because they are superior to any other product of this kind on the market. They are easy to use, give consistent results, economical and are suitable for a wide range of applications. The flow slides have without a doubt transformed my labs research and made many new experiments possible. Well done ibidi! I am indeed extremely happy with the ibidi products.”
Dr. Thomas A.J. McKinnon BSc, PhD
Post Doctoral Research Associate
Department of Hematology
Imperial College London
Prof. Dr. Markus Sauer, University of Wuerzburg, Germany
„ibidi slides are compelling!
We tested the open μ-Slides with 8 wells for live-cell super-resolution imaging by dSTORM with SNAP-tags. The quality of the super-resolved images was impressive.“
Prof. Dr. Markus Sauer
Biotechnology & Biophysics
Prof. Dr. Stefan Zahler, University of Munich, Germany
„Using the „angiogenesis slide“ from ibidi is the only possibility that I know for achieving a consistently good optical quality in the tube formation assay, and saving Matrigel at the same time.
In comparison to other systems, the heating stage from ibidi shows superior thermal stability and enables you to work with high humidity (>80%). This is not possible with other systems but is indispensable for long-term studies.“
Prof. Dr. Stefan Zahler
Munich Center for System-Based Drug Research
Jason Jacoby, University of Illinois, USA
„Actually, ibidi dishes have single-handedly revolutionized my science. The cells that I use (horizontal retinal cells from fish) are extremely intolerant to being plated on any form of glass, even if the glass is treated with several different compounds. Because of this issue, I was only able to perform fluorescent microscopy on upright confocal microscopes; they allowed us to plate our cells on plastic dishes and image from above. Now, with the ibidi dishes, we are able to use better quality inverted microscopes and higher powered objectives. Our results have proven strong, and we have mentioned the power of ibidi products in our most recent publication.
I am so happy that I learned of your great products through a colleague. Microscopy will never be the same for our laboratory. So far I have purchased 3 boxes of 35mm ibiTreat high dishes. Thank you very, very much.”
Jason Jacoby PhD Candidate
Laboratory of Neuroscience
University of Illinois at Chicago
Prof. Dr. Klaus Palme, University of Freiburg, Germany
„The cooperation with ibidi made it possible to provide cell culture systems with a unique optical quality. The use of ibidi slides allowed for image acquisition in the necessary optical resolution, so that development processes in living proteoplasts of the model plant Arabidopsis thaliana could be explored. The µ-Slides allow monitoring of developmental processes in highest resolution. This enables us to use genetic techniques for recording and controlling the necessary processes of organ development.”
Prof. Dr. Klaus Palme
Institute for Biology II
Freiburg Institute for Advanced Studies FRIAS – School of Life Sciences