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Tish Essebier
Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia
Bead sprouting assay shows irregular blood vessel formation of human umbilic venous endothelial cells (HUVECs). Cells were transduced with an mScarlet-tagged lentiviral plasmid (magenta), embedded into a fibrin gel in an ibidi µ-Plate 24 Well, and co-cultured with lung fibroblasts for 7 days. The actin cytoskeleton was stained with phalloidin (green) and nuclei were stained with DAPI (blue). The image was taken using a Zeiss LSM 710 Meta confocal scanner with a 10x objective.
Published in the 2022 ibidi Calendar.
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