Stage Top Incubator
Neurobiology
Istituto Nanoscienze @NEST, Consiglio Nazionale delle Ricerche, Pisa, Italy
Confocal microscopy image of primary murine cortical neurons (Div3) cultured on 10 µm-width lines of Poly-L-lysine (PLL) conjugated to fluorescein (green). The line pattern has been developed by soft lithography on glass slides. Neurons were immunostained for Map-2 (yellow), actin fibers were stained with phalloidin (red), nuclei with DAPI (blue). The image was acquired using a Zeiss LSM700 confocal microscope with a 20x objective.
Published in the 2021 ibidi Calendar.
