Angiogenesis Assays

Which cell density is optimal for my tube formation experiment?
During which timeframe should I observe the cells when performing a tube formation assay?
Do I need a live cell imaging setup to take pictures of the tube formation assay every hour?
Is it possible to cultivate cells inside a gel matrix using the ibidi µ-Slide 15 Well 3D?
Should I use gels with phenol red or phenol red-free gels for my tube formation experiments?
Is it necessary to put the µ-Slide 15 Well 3D into the humid chamber of the incubator for the gel polymerization?
Does the fetal bovine serum (FBS) / fetal calf serum (FCS) concentration affect the rate of tube formation and degradation in tube formation experiments?
Do you recommend using growth factor-reduced Matrigel® for tube formation assays?
We want to use growth factor-reduced Matrigel® and serum-free media plus 50 ng/ml VEGF. Is this enough to stimulate the tube formation?
Do you have any experience with gels other than Matrigel® in tube formation assays?
What would be a suitable positive and negative control for tube formation experiments?
Is there a recommended staining protocol for the analysis of tube formation experiments?
Do I have to equilibrate the ibidi angiogenesis labware to 37°C before starting the tube formation experiment?
After completing the experiment, can the ibidi µ-Slide 15 Well 3D and the ibidi µ-Plate 96 Well 3D be reused?
Is the ibidi µ-Slide 15 Well 3D available in a 96 well format?

After completing the experiment, can the ibidi µ-Slide 15 Well 3D and the ibidi µ-Plate 96 Well 3D be reused?

No, the ibidi µ-Slide 15 Well 3D and ibidi µ-Plate 96 Well 3D are disposables, which are for single-use only.

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