Cells on gel matrices can be used to monitor their ability to form new vascular structures. Tube formation assays are carried out by seeding single cells onto the gel matrix and observing characteristic patterns. To carry out sprouting assays, either spheroids or pieces of tissue, e.g., from the aorta, are placed onto the gel matrix. Both these types of assays require that the underlying gel layer be of uniform thickness.
µ-Plate Angiogenesis 96 well vs. Standard 96 well Plate
µ-Plate Angiogenesis 96 Well
Planar air-liquid interface: good phase contrast all over the observation area
Planar gel surface: all cells are in one optical plane
Volume of Matrigel: 10 µl
Meniscus on air-liquid interface: poor phase contrast in most of the observation area
Mensicus on the gel surface: not possible to focus on all cells simultaneously
Sam Noppen, Rega Institute for Medical Research, Leuven, Belgium
"I used the ibidi µ-Plate Angiogenesis 96 Well for bioluminescence measurements. The plate performed exceptionally well for this specific application, as it reduced my sample volume drastically and the optical quality was just superb."
Sam Noppen Rega Institute for Medical Research, KU Leuven Leuven Belgium