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3 Well Chamber, removable

3 Well Chamber, removable View larger

Removable 3 Well silicone cultivation chamber for cell culture and immunofluorescence staining

  • Perfect for immunofluorescence staining and long-term sample storage
  • Cost effective classic immunofluorescence assays
  • Large growth area combined with a small staining volume (especially 3 Well Chamber, removable)
  • Suitable for use with upright and inverted microscopes

More details

microscopy glass slide, sterilized
15

Buy 4 - Get 1 Free

Quantity Discount
5 20%

Product Details

Applications:

  • Immunofluorescence assays using standard coverslip techniques
  • Long-term sample storage
  • 3 fold sample preparation of adherent cells and tissue samples

Technical Features:

  • Self-adhesive silicone gasket mounted on a standard glass slide
  • Biocompatible silicone material
  • Non-fluorescent glass slide with frosted ends for easy handling and labeling
  • Suitable for use with upright and inverted microscopes, after staining and mounting with a coverslip
  • Staining volume reduction by glass coverslip
  • Compatible with all common mounting media
  • Optional: The gasket may be transferred to any flat clean surface for cell cultivation

Specifications:

Number of wells 3
Dimensions of wells (w x l x h) in mm 16.5 x 16.5 x 8.0
Volume per well 1100 µl (150 µl*)
Growth area per well 1.66 cm²
Coating area per well 3.73 cm²
Material Biocompatible silicone
Suitable coverslip size 24 mm x 60 mm
Bottom Glass slide (26 x 76 x 1 mm)

* Volume reduction for staining when using a 15 mm glass coverslip (Cat. No. 10815)

Standard Procedure 3 Well | 8 Well | 12 Well Chamber, removable:

Low Volume Staining Procedure 3 Well Chamber, removable:

Staining Protocol:

Supporting Material

User Comments

Petra Weber, Aalen University, Optical Engineering, Aalen, Germany

"We have tested the Removable Chambers (3 Well and 8 Well) and were very happy. Due to our very sensitive TIRF microscopy with an upright microscope we need appropriate glass slides with removable chambers. We tested adherent HeLa cells to detect Förster Resonance Energy Transfer between EGFR-CFP and Grb2-YFP with Fluorescence Lifetime Imaging. In particular for the activation or inhibition of intracellular signaling pathways, we need the subdivisions on the object slide. It was very easy to remove the chambers. Thank you very much for the possibility to test the chambers for free. I am sure I will use them again."

Petra Weber
Aalen University, Optical Engineering
Aalen, Germany

https://www.hs-aalen.de/de

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