Step 2: remove insert after cultivation Step 3: overlay patches with culture medium
Wound Healing / Migration
The Culture-Insert 4 Well allows for wound healing and migration assays with four cell-free gaps with a 0.5 mm gap width, and one center area with a 1 mm diagonal distance. Compared to the 2 well version, more cells can be analyzed and a higher throughput is realized. Two cell-free gaps create a technical replicate in the very same well.
Two Conditions of Wound Healing / Migration
With the Culture-Insert 4 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).
Four Conditions for Co-Cultivation / Invasion
By seeding up to four different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.
Invasion and co-cultivated cells can be imaged by using high-resolution fluorescence microscopy.
Ali Alhoshani, King Saud University, Riyadh, Saudi Arabia
“The ibidi Culture-Insert 2 Well 24plate was unbelievably helpful when I wanted precise and consistent results for wound healing assay. It saved my lab so much time and effort. The web-based quantitative image analysis feature is a huge plus. Thank you for such useful products. "
Ali Alhoshani King Saud University Department of Pharmacology and Toxicology Riyadh Saudi Arabia