µ-Dish 35 mm, high Glass Bottom

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µ-Dish 35 mm, high Glass Bottom

A 35 mm imaging dish with a glass bottom, suitable for use in TIRF and single molecule applications

Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass (# 1.5H)

Lid with lock position, which minimizes evaporation

Suitable for DIC, when used with the special DIC Lid

Also available with a relocation grid: Grid-50 / Grid-500
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A 35 mm imaging dish with a glass bottom, suitable for use in TIRF and single molecule applications

Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass (# 1.5H)
Lid with lock position, which minimizes evaporation
Suitable for DIC, when used with the special DIC Lid
Also available with a relocation grid: Grid-50 / Grid-500

More details

Surface Modification

#1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized

Pcs./Box

Product Details

Applications

Cultivation and microscopy of cell cultures
Fluorescence Correlation Spectroscopy (FCS)
TIRF and single molecule applications

Specifications

Ø µ-Dish	35 mm
Volume	2 ml
Growth area	3.5 cm²
Coating area using 400 µl	4.1 cm²
Ø observation area	21 mm
Height with / without lid	14/12 mm
Bottom	Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Features

Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
Standard format dish with a 35 mm diameter and high walls
May require coating to promote cell attachment

Experimental Examples

Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.

Fluorescence microscopy of fixed rat fibroblasts on a Glass Coverslip #1.5H. F-actin filaments are stained with phalloidin (green), nuclei are stained with DAPI (blue).

Detailed Comparison ibidi Dishes^{35 mm}

	µ-Dish^{35 mm, high} (Polymer Coverslip, ibiTreat / Uncoated) 81156, 81151	µ-Dish^{35 mm, high} Glass Bottom 81158	Glass Bottom Dish^{35 mm} 81218-200 81218-800
Bottom Thickness	180 µm (+10/-5 µm)	170 µm (+/-5 µm)	170 µm (+20/-10 µm)
Bottom Material	#1.5 Polymer coverslip (ibidi)	#1.5H glass coverslip (D 263 M Schott high precision glass)	#1.5 glass coverslip (D 263 M Schott high precision glass)
Lid	Lid lock for minimal evaporation and safe handling	Lid lock for minimal evaporation and safe handling	Standard
Surfaces	ibiTreat (tissue culture treated), Uncoated (hydrophobic)	Uncoated glass	Uncoated glass
Gas permeable	Yes	No	No
Packaging	Sterile, single-packed	Sterile, single-packed	Sterile, packed at 10 pcs.
Quantity	60 pcs./box	60 pcs./box	200/800 pcs./box
Free Sample	Yes	Yes	Yes

Microscopy Applications

High resolution microscopy¹
Confocal	+	+	+
Brightfield, Phase Contrast, DIC	+	+	+
Widefield Fluorescence	+	+	+
TIRF	-	+	-
Super-Resolution microscopy²
STED, STORM, etc.	-	+	-

Optical Properties

Refractive Index (n_D 589 nm)	1.52	1.52	1.52
Abbe number	55	56	56
Autofluorescence	Low	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)	High (ultraviolet restrictions)
Birefringence (DIC)	Low (DIC compatible³)	Low (DIC compatible³)	Low (DIC compatible³)

Note:

¹ High resolution microscopy = high magnification (40x - 100x) and high resolution microscopy including widefield or confocal techniques (e.g., brightfield, DIC, fluorescence). The resolution is limited to ~200 nm by the diffraction limit. Does not include Super-Resolution.
Resolution is described as a function to discriminate 2 dots from each other; it is dependent on wavelength and numerical aperture. It is physically limited by Abbe’s law which can only be overcome by Super-Resolution microscopy.

² Super-Resolution microscopy = additional resolution by overcoming the diffraction limit with 63x - 100x oil immersion objectives. Super-Resolution provides a resolution below 200 nm, down to 5-20nm by computational (PALM, SIM, STORM, RESOLFT) and one optical Super-Resolution technology (STED). TIRF is not a Super-Resolution technology.

³ DIC Compatibility = The lids are not DIC compatible. Special glass DIC Lids for the µ-Dishes are available separately.

Supporting Material

Instructions

Instructions µ-Dish^{35 mm, high} Glass Bottom (PDF)

Application Notes

AN 08: Cell Culture Coating (PDF)

FAQ

User Comments

Contu Raluca, National Center of Neurology and Psychiatry, Tokyo, Japan

"We often use ibidi glass bottom dishes for live imaging of cultured cells. We were able to get very good images of protein delocalization, as well as of nucleic acids taken up by living cells. The optical quality of the bottom of the dishes is extraordinary. Actually, two of our papers for which we used ibidi µ-Dishes and μ-Slides were published in 2017:

Contu et al. 2017 J Cell Sci. http://jcs.biologists.org/content/130/17/2843.long

Takahashi et al. 2017 RNA Biology http://www.tandfonline.com/doi/full/10.1080/15476286.2017.1302641

Contu Raluca
National Center of Neurology and Psychiatry
Tokyo
Japan