1 High resolution microscopy = high magnification (40x - 100x) and high resolution microscopy including widefield or confocal techniques (e.g., brightfield, DIC, fluorescence). The resolution is limited to ~200 nm by the diffraction limit. Does not include Super-Resolution. Resolution is described as a function to discriminate 2 dots from each other; it is dependent on wavelength and numerical aperture. It is physically limited by Abbe’s law which can only be overcome by Super-Resolution microscopy.
2 Super-Resolution microscopy = additional resolution by overcoming the diffraction limit with 63x - 100x oil immersion objectives. Super-Resolution provides a resolution below 200 nm, down to 5-20nm by computational (PALM, SIM, STORM, RESOLFT) and one optical Super-Resolution technology (STED). TIRF is not a Super-Resolution technology.
³ DIC Compatibility = The lids are not DIC compatible. Special glass DIC Lids for the µ-Dishes are available separately.
Contu Raluca, National Center of Neurology and Psychiatry, Tokyo, Japan
"We often use ibidi glass bottom dishes for live imaging of cultured cells. We were able to get very good images of protein delocalization, as well as of nucleic acids taken up by living cells. The optical quality of the bottom of the dishes is extraordinary. Actually, two of our papers for which we used ibidi µ-Dishes and μ-Slides were published in 2017: