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25 Culture-Inserts 3 Well for self-insertion

25 Culture-Inserts 3 Well for self-insertion View larger

Optimized for inverted microscopy Suitable for adherent cells Suitable for wound healing and migration assays

3 well silicone insert with two defined cell-free gaps, suitable for wound healing, migration assays, 2D invasion assays, and co-cultivation of cells

  • Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
  • Reproducible experiments owing to: two defined 500 µm cell free gaps, no leakage during cultivation, and no material being left behind after the insert’s removal
  • Suitable for 3 experiments in parallel
  • 25 pieces in a transport dish
  • Used for self-insertion into 6 or 12 well plates, or other formats

Not to be used without transferring the Culture-Inserts

More details

25

Product Details

Specifications

Number of wells 3
Outer dimensions 8.4 x 12.15 x 5 mm (w x l x h)
Volume per well 70 µl
Growth area per well 0.22 cm²
Coating area per well 0.82 cm²
Width of cell free gap 500 µm +/- 100 µm
Material Biocompatible silicone
Bottom No bottom - sticky underside

Technical Features

  • Culture-Insert 3 Well in a transport petri dish for self-insertion
  • Biocompatible silicone material with adhesive underside
  • Transferable to any flat and clean surface
  • Has to be placed in an appropriate cell culture vessel before use, such as the µ-Slide 2 Well, 6 well plates, or 12 well plates
  • Clean surface after insert removal without remaining material

Principle for Wound Healing and Migration Assays

Various Applications

Wound Healing / Migration

Wound healing and migration assays are done by seeding cells into the Culture-Insert 3 Well. After cell attachment, two cell-free gaps are created in which the cell migration can be visualized. Compared to the 2 well version, more cells can be analyzed and a higher throughput is achieved. Two cell-free gaps create a technical replicate in the very same plate or dish.

Two Conditions of Wound Healing / Migration

With the Culture-Insert 3 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).

Co-Cultivation / Invasion

By seeding two different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.

Invasion and co-cultivated cells can be imaged by high-resolution fluorescence microscopy.

Visualization of Cell Migration

Live cell imaging of a wound healing and migration assay using the MCF7 cell line. Objective lens 10x, phase contrast microscopy.

Supporting Material

Movies

User Comments

Ali Alhoshani, King Saud University, Riyadh, Saudi Arabia

“The ibidi Culture-Insert 2 Well 24 plate was unbelievably helpful when I wanted precise and consistent results for wound healing assay. It saved my lab so much time and effort. The web-based quantitative image analysis feature is a huge plus. Thank you for such useful products. "

Ali Alhoshani
King Saud University
Department of Pharmacology and Toxicology
Riyadh
Saudi Arabia

Henrike Rebl, University Medicine, Rostock, Germany

"I have tested the Culture-Insert 2 Well and 3 Well and they worked quite nicely. I will definitely use the ibidi Culture-Inserts and µ-Dishes again because they are so easy to use and they work great for fluorescence microscopy."

Henrike Rebl
University Medicine
Rostock, Germany
https://www.med.uni-rostock.de

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