Heating Stages for Live Cell Imaging - No Condensation
The independently controlled, heated glass lid of the ibidi Heating & Incubation System solves the problem of condensation in live cell imaging.
By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.
Components of the ibidi Heating & Incubation System, Universal Fit
ibidi Temperature Controller
Provides excellent temperature stability within the incubation chamber
Four control channels allow to connect a heated plate, a heated lid, and two additional heated components (e.g., an objective heater)
Temperature stability: ± 0.05 °C
Temperature uniformity across the insert: ± 0.5 °C
Temperature range: Room temperature - 45 °C
Control channels: 4
TempControl software for remote control and data recording included
External temperature sensor included
Adjustable alarm function included
Analog output for extreme noise reduction optional
Heated Plate in a Multi-well Format for 1 Chamber
Can be used with any inverted microscopy system
Fits into both manual and automated stages
Can be placed into any regular multi-well frame that comes with your microscope
The heated lid also comes with a gas inlet. The ibidi Gas Incubation System upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications, including high resolution, TIRF, and confocal microscopy.
The ibidi Gas Incubation System is suitable for various experimental conditions (e.g., pH or hypoxia). It is available in two versions: Gas Mixer for CO2, Gas Mixer for CO2 and O2.
Live Cell Imaging Under Flow Conditions
The ibidi Heating & Incubation System can be adapted for live cell imaging under flow conditions. Connect the ibidi Pump System with the ibidi Heating System, Universal Fit, by using the Heating Insert Adapter for perfusion assays. This creates the perfect environment for long-term cell studies (several days) under flow conditions.
Overview of the Heating and Incubation System, Universal Fit
ibidi Heating System for Temperature Control of Multi-Well Plates
ibidi has developed a Heating System for the use with K-Frame Stages or the Nikon Ti-S-E and Ti-S-ER Motorized Stage which hold multi-well plates. Thus, this system permits the user to control the temperature of samples held in multi-well plates.
You can also obtain additional customer-specific solutions upon request.
"For my research, I need to take time-lapse images of mouse embryos developing from the 1 cell stage all the way to 5-day blastocyst. Anyone who has worked with mouse embryos knows they are incredibly sensitive and delicate, so we required an extremely stable environment over several days. I struggled for months with a different system, but the ibidi Heating Stage worked very quickly. I was immediately impressed with the quality. Calibration with the included thermocouple was easy and accurate. Now I get consistent development every time, and the percentage of embryos developing to blastocysts is the same as the control incubator! Terrific product, thanks ibidi.”
"The ibidi µ-Slides Chemotaxis 2D and 3D have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.
I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"
Prof. Dr. Stefan Zahler, University of Munich, Germany
„Using the „angiogenesis slide“ from ibidi is the only possibility that I know for achieving a consistently good optical quality in the tube formation assay, and saving Matrigel at the same time.
In comparison to other systems, the heating stage from ibidi shows superior thermal stability and enables you to work with high humidity (>80%). This is not possible with other systems but is indispensable for long-term studies.“
Prof. Dr. Stefan Zahler, Pharmaceutical Biology, Munich Center for System-Based Drug Research, Ludwig-Maximilians-University, Germany www.pharmbiol.cup.uni-muenchen.de