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µ-Slide 8 Well Grid-500
An open µ-Slide (chambered coverslip) with 8 wells and an imprinted 500 µm cell location grid for use in immunofluorescence and high-end microscopy
- Ideal for locating cells or cell clusters
- Observation of the sample through the polymer coverslip bottom using high resolution microscopy
- Cost-effective experiments using small numbers of cells and low volumes of reagents
Product Details
Applications
- Locating cells or cell clusters, e.g., transfected cells for clone picking
- Counting events per defined area (e.g., for calculating transfection efficiency)
- Providing a reference structure for cell movements
- Cultivation and microscopy of cell cultures
- Transfection assays
- Fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Suitable for DIC, when used with a DIC lid
Specifications
Geometry of the Grid-500
| Number of view fields | 100 |
| Repeat distance | 500 µm |
| Groove width | 5 µm +/- 1 µm |
| Groove depth | < 5 µm |
Geometry of the µ-Slide 8 Well
| Number of wells | 8 |
| Dimensions of wells (w x l x h) | 9.4 x 10.7 x 6.8 mm |
| Volume per well | 300 µl |
| Total height with lid | 8 mm |
| Growth area per well | 1.0 cm² |
| Coating area per well | 2.20 cm² |
| Bottom: ibidi Polymer Coverslip | |
Technical Features
- Grid with a 500 µm repeat distance
- Lettered and numbered fields (A-K; 1-10)
- Open µ-Slide (chambered coverslip) with 8 independent wells
- Grid clearly visible in phase contrast and bright field microscopy; slightly visible in fluorescence mode
- Excellent optical quality imaging chamber for high-end microscopy
- Compatible with staining and fixation
- ibiTreat surface for optimal cell adhesion
- Biocompatible plastic material — no glue, no leaking
Layout Grid-500
Supporting Material
User Comments
Astrid König, Senckenberg Museum of Natural History, Görlitz, Germany
We use the μ-Slide 8 Well Grid-500 for tracking experiments of tardigrades and nematodes. We are really thrilled about the product. The scaling of the slide helps us very much in evaluating the movement of the animals during the experiment.
Please find more details at:
http://onlinelibrary.wiley.com/doi/10.1111/zoj.12435/full
Astrid König
Senckenberg Museum of Natural History
Görlitz, Germany
Sofia Oke, University of Toronto, Department of Immunology, Toronto, Canada
“I have had the chance to try the µ-Slide 8 Well Grid-500 and have been very impressed. I have been using the slides to perform assays with 3D organoid cultures. The µ-Slide 8 Well Grid-500 was very useful and easy for me to monitor my organoids as they grew. I was performing an assay where I irradiated my organoids and monitored recovery. I am very impressed with these products and will be sure to keep in touch if I once again need them in the months to come.
Sofia Oke
University of Toronto, Department of Immunology
Toronto, Canada
