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µ-Dish 35 mm, high Grid-500 Glass Bottom
A 35 mm imaging dish with a glass bottom and an imprinted 500 µm cell location grid
- Ideal for locating cells or cell clusters
- Easy cell counting per defined area
- Glass coverslip for TIRF and single molecule applications
Product Details
Applications
- Locating cells or cell clusters, e.g., transfected cells for clone picking
- Counting events per defined area (e.g., when calculating transfection efficiency)
- Providing a reference structure for cell movements
- Treatment of distinct single cells, such as in microinjection
- Following axon growth on a defined scale bar
Specifications
Geometry of the µ–Dish 35 mm, high Glass Bottom
| Diameter dish | 35 mm |
| Volume | 2 ml |
| VGrowth area | 3.5 cm2 |
| Coating area using 400 µl | 4.1 cm2 |
| Diameter observation areal | 21 mm |
| Height with / without lid | 14 mm / 12 mm |
| Bottom | Glass coverslip No. 1.5H |
Geometry of the Grid-500
| Number of squares | 400 |
| Repeat distance | 500 µm |
| Groove width | 20 µm (± 5 µm) |
| Groove depth | < 5 µm |
Technical Features
- Grid with 500 µm repeat distance
- Lettered and numbered 4 x 100 squares (A-U; 1-20)
- Grid and cells in one focal plane
- Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
- Lid with lock position, which minimizes evaporation
- May require coating to promote cell attachment
- Grid-500 also available with ibidi Polymer Coverslip bottom in µ-Dish 35 mm, high Grid-500 or µ-Slide 8 Well Grid-500
Layout Grid-500 Glass Bottom
Experimental Example
Triple immunofluorescence of MDCK cells
Red: mitochondria, stained with MitoTracker™ Red CMXRos
Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin
Blue: nuclei, stained with DAPI
ibidi Polymer Coverslip vs. ibidi Glass Bottom
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| Optical properties | ||
| Refractive index (nD 589 nm) | 1.52 | 1.52 |
| Abbe number | 56 | 55 |
| Thickness | #1.5 (180 µm) | #1.5H (170 µm) |
| Material | Microscopy plastic | D 263M Schott borosilicate glass |
| Autofluorescence | Low | Low |
| Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
| Birefringence (DIC) | Low (DIC compatible*) | Low (DIC compatible*) |
| Other aspects | ||
| Surface modifications | ibiTreat – tissue culture treated Uncoated – hydrophobic |
Only glass |
| Protein coatings | Compatible | Compatible |
| Gas permeable | Yes | No |
| Material flexibility | High | Low |
| Breakable | No | Yes |
| Applications | Fluorescence microscopy | TIRF and single photon |
* Special glass DIC Lids are available separately
Supporting Material
Instructions
Application Notes
Product Flyer
FAQ
User Comments
James J. Faust and David G. Capco, Arizona State University, Tempe, USA
“ibidi has made the tedious process of correlated light- and scanning electron microscopy remarkably simplified with their µ-Dishes 35mm, high Grid-500 Glass Bottom. This product combines all of the essential elements for high quality imaging: optically flawless glass essential for high resolution light microscopy, media reservoirs suited for a variety of imaging systems, and a grid system to rapidly relocate specimens in the scanning electron microscope. This product has made the difficult easy.”
James J. Faust and David G. Capco
Molecular and Cellular Biosciences
Arizona State University
Tempe, AZ
USA
