For pricing and online shopping, please log in.
Product filter
Product filter
Glass Bottom
-
Application
- Live Cell Imaging
- Immunofluorescence
- Wound Healing and Migration
- Chemotaxis
- Angiogenesis
- Cell Culture Under Flow
- Optical Oxygen Measurement
- Counting and Locating Cells
- Screening | High Throughput
- Correlative Light and Electron Microscopy CLEM
- Super-Resolution Microscopy
- Impedance-Based Cell Assays
- LifeAct Actin Visualization
- Membrane Fusion | Transfection
- Labware
- Instruments
- Cells | Reagents | Services
- Software and Image Analysis
Top sellers
-
µ-Slide 8 Well
An open µ-Slide (chambered coverslip) with 8 wells for use in...
-
25 Culture-Inserts 2 Well for self-insertion
2 well silicone insert with a defined cell-free gap, suitable for wound...
-
µ-Dish 35 mm, high
A 35 mm imaging dish with an ibidi Polymer Coverslip Bottom, used in...
-
µ-Slide I Luer
Channel slides with different heights, volumes, and coatings specially...
µ-Slide 2 Well Glass Bottom
Open µ-Slide with 2 wells with a glass bottom, suitable for use in TIRF and single molecule applications
- Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
- Cost-effective experiments using small numbers of cells and low volumes of reagents
Buy 4 - Get 1 Free
| Quantity | Discount |
|---|---|
| 5 | 20% |
Product Details
Applications:
- Cultivation and microscopy of cell cultures
- TIRF and single molecule applications
Technical Features
- Open µ-Slide with 2 independent wells
- Bottom made from D 263 M Schott glass, No. 1.5H
(170 +/- 5 µm) - May require coating to promote cell attachment
- Also available in a Ph+ version: intermediate plate in each well to avoid meniscus formation
- Also available as a µ-Slide 2 Well with an ibidi Polymer Coverslip Bottom for superior cell growth
Specifications:
| Number of wells | 2 |
| Dimensions of wells (w x l x h) in mm |
21.2 x 23.3 x 9.3 |
| Volume per well | 1500 µl |
| Total height with lid | 10.8 mm |
| Growth area per well | 4.8 cm² |
| Coating area per well | 7.5 cm² |
| Bottom: | Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm |
ibidi Polymer Coverslip vs. ibidi Glass Bottom
|
|
|
|
| Optical properties | ||
| Refractive index (nD 589 nm) | 1.52 | 1.52 |
| Abbe number | 56 | 55 |
| Thickness | #1.5 (180 µm) | #1.5H (170 µm) |
| Material | Microscopy plastic | D 263M Schott borosilicate glass |
| Autofluorescence | Low | Low |
| Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
| Birefringence (DIC) | Low (DIC compatible*) | Low (DIC compatible*) |
| Other aspects | ||
| Surface modifications | ibiTreat – tissue culture treated Uncoated – hydrophobic |
Only glass |
| Protein coatings | Compatible | Compatible |
| Gas permeable | Yes | No |
| Material flexibility | High | Low |
| Breakable | No | Yes |
| Applications | Fluorescence microscopy | TIRF and single photon |
* Special glass DIC Lids are available separately
Supporting Material
Instructions
Instructions µ-Slide 2 Well Glass Bottom (PDF)
Application Notes
AN 08: Cell Culture Coating (PDF)
