µ-Slide 2 Well Glass Bottom

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µ-Slide 2 Well Glass Bottom

Open µ-Slide with 2 wells with a glass bottom, suitable for use in TIRF and single molecule applications

Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass

Cost-effective experiments using small numbers of cells and low volumes of reagents
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Open µ-Slide with 2 wells with a glass bottom, suitable for use in TIRF and single molecule applications

Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
Cost-effective experiments using small numbers of cells and low volumes of reagents

More details

Surface Modification

#1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized

Pcs./Box

Product Details

Applications

Cultivation and microscopy of cell cultures
TIRF and single molecule applications

Specifications

Outer dimensions in mm (w x l)	25.5 x 75.5
Number of wells	2
Dimensions of wells (w x l x h) in mm	21.6 x 23.8 x 9.3
Volume per well	1500 µl
Total height with lid	10.8 mm
Growth area per well	5.1 cm²
Coating area per well	7.5 cm²
Bottom	Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Features

Open µ-Slide with 2 independent wells
Bottom made from D 263 M Schott glass, No. 1.5H
(170 +/- 5 µm)
May require coating to promote cell attachment
Also available in a Ph+ version: intermediate plate in each well to avoid meniscus formation
Also available as a µ-Slide 2 Well with an ibidi Polymer Coverslip Bottom for superior cell growth

Experimental Example

Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.

ibidi Polymer Coverslip vs. ibidi Glass Bottom

	ibidi Polymer Coverslip	ibidi Glass Bottom
Optical properties
Refractive index (n_D 589 nm)	1.52	1.52
Abbe number	56	55
Thickness	#1.5 (180 µm)	#1.5H (170 µm)
Material	Microscopy plastic	D 263M Schott borosilicate glass
Autofluorescence	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)
Birefringence (DIC)	Low (DIC compatible*)	Low (DIC compatible*)
Other aspects
Surface modifications	ibiTreat – tissue culture treated Uncoated – hydrophobic	Only glass
Protein coatings	Compatible	Compatible
Gas permeable	Yes	No
Material flexibility	High	Low
Breakable	No	Yes
Applications	Fluorescence microscopy	TIRF and single photon

* Special glass DIC Lids are available separately

Supporting Material

Instructions

Instructions µ-Slide 2 Well Glass Bottom (PDF)

Application Notes

AN 08: Cell Culture Coating (PDF)

FAQ

User Comments

Serene Gwee, National University of Singapore, Singapore

"I started using the Glass Bottom chambered slides with my time-lapse imaging of zebrafish embryos and they have been very useful and applicable for my experiments."

Sze Ling Gwee
National University of Singapore
Department of Biological Sciences
Singapore

http://www.dbs.nus.edu.sg/