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Gas Incubation Systems
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- Live Cell Imaging
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ibidi Gas Incubation System for CO2 and O2
A gas mixer that upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications with CO2, O2, and humidity control
- Ideal for live cell imaging applications - full incubator conditions on the microscope
- Stable gas incubation without evaporation (patent pending technology)
- Suitable for various experimental conditions (e.g., pH or hypoxia)
- Fully compatible with the ibidi Heating Systems and stage top incubators of other suppliers
- System includes: ibidi CO2 and O2 Gas Mixer, Humidifying Column
System includes:
 ibidi Gas Mixer |
 Humidifying Column |
 Optional: Air Pressure Generator |
Product Details
Applications:
- Live cell imaging that requires incubator conditions on the microscope, for example:
- Chemotaxis
- Angiogenesis
- Cell migration, proliferation, wound healing
- Cell culture under flow - Imaging applications with extra focus stability, for example:
- TIRF
- Confocal microscopy - Anaerobic conditions or oxidative stress
Technical Features:
- Precise and reliable gas incubation for O2 and CO2
- Active and fast humidification - no evaporation
- Uses pressurized air to create the gas flow – no vibrations
- Optional air pressure generator available (for when pressurized air is not obtainable)
The ibidi Gas Incubation System provides both humid and CO2-rich air for stage-top incubators like the ibidi Heating System. The gas mixture is continuously flushed through the stage top incubator, ensuring a maximum humidity and an optimal pH for CO2-buffered liquids.
Spheroid of breast cancer cells with living cells (green) and apoptotic center (red) due to oxygen depletion.
Components of the ibidi Gas Incubation System
ibidi Gas Mixer for CO2 and O2
- Precise and reliable gas incubation
- Active and fast humidification without evaporation (patent pending technology)
- Suitable for various experimental conditions (e.g., pH or hypoxia)
- Fully compatible with the ibidi Heating Systems
- Uses pressurized air to create the gas flow – no vibrations
- Optional air pressure generator available (for when pressurized air is not obtainable)
Humidifying Column
- Humidifies the outgoing CO2 / O2 / air mix to minimize sample evaporation
- Active humidity control inside the attached ibidi Heating System
ibidi Heating System
The ibidi Gas Incubation System upgrades your ibidi Heating System to a complete stage top incubator.
Oxygen Conditions in Various Surroundings
| Location/Tissue | Oxygen Partial Pressure p(O2) | Corresponding O2 in Cell Culture Gas Mix (%) |
| Inspired air | 21 kPa (= 160 mm Hg) | 21% |
| Alveolus | 14 kPa (= 104 mm Hg) | 14% |
| Arterial blood | 14 kPa (= 104 mm Hg) | 14% |
| Veneous blood | 5 kPa (= 40 mm Hg) | 5% |
| Interstitial fluid | 5 kPa (= 40 mm Hg) | 5% |
| Healthy tissue | 2–3 kPa (= 15- 23 mm Hg) | 2–3% |
| Tumor without blood vessels | ||
| Outside | 1 kPa (= 7.5 mm Hg) | 1% |
| Center | 0 kPa (= 0 mm Hg) | 0% |
| Normoxia conditions | 2–11 kPa (= 15–82 mm Hg) | 2–11% |
| Hypoxia conditions | 0–1 kPa (= 0–7.5 mm Hg) | 0–1% |
| Cell culture incubator (5% CO2) | 20 kPa (= 150 mm Hg) | 20% |
Overview of the ibidi Heating & Gas Incubation System, CO2 and O2 Control
Gas Supply (Input)
Specifications:
| Gas Supply (Input) | |
| Pressurized air | 1 bar optimum / 14.5 psi (0.8–1.2 bar / 11.6–17.4 psi) |
| CO2 | 1 bar optimum / 14.5 psi (0.8–1.2 bar / 11.6–17.4 psi) |
| N2 | 1 bar optimum / 14.5 psi (0.8–1.2 bar / 11.6–17.4 psi) |
| Tubing for all gases (supplied with the system) | PUR/PUN tubing 4 mm inner diameter 6 mm outer diameter 1 mm wall thickness |
ibidi Gas Mixer
Specifications:
| CO2 Control | |
| CO2 control range | 0%–15% |
| Resolution | 0.1% |
| O2 Control | (only ibidi Gas Incubation System for CO2 and O2) |
| O2 control range | 1%–21% (Settings below 1% are less accurate, a typical minimum value in an ibidi Heating Chamber is 0.5% O2) |
| Resolution | 0.1% |
| Gas Flow | |
| Control range | 0–20 l/h |
Humidifying Column
Specifications:
| Humidity Control | |
| Control range | 20%–99% (relative humidity) |
| Resolution | 0.1% |
| Maximal volume | 130 ml ultrapure water |
| Minimal volume | 50 ml ultrapure water |
ibidi Heating System
The ibidi Gas Incubation System is compatible with:
Supporting Material
Instructions
IncuControl Software
Product Flyer
- Product Flyer Heating & Incubation Systems (PDF)
- Important Handling Information for the ibidi Gas Incubation System (PDF)
FAQ
User Comments
Loïc Dupré, INSERM, Toulouse, France
"The ibidi µ-Slides Chemotaxis 2D and 3D have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.
I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"
Loïc Dupré PhD, INSERM UMR 1043, Purpan University Hospital, Toulouse, France
http://www.cptp.inserm.fr/
